Culturing substrates influence the morphological, mechanical and biochemical features of lung adenocarcinoma cells cultured in 2D or 3D
| Autor: | Suzanne P. Jarvis, Jason I. Kilpatrick, Dania Movia, Adriele Prina-Mello, Alan P. Bell, Baiyun Liu, Melissa Anne Tutty, Yuri Volkov, Namrata Jain |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lung Neoplasms Confocal Cell Culture Techniques Adenocarcinoma of Lung Enzyme-Linked Immunosorbent Assay Docetaxel Adenocarcinoma Biology Biophysical Phenomena Substrate Specificity 03 medical and health sciences In vivo Tumor Cells Cultured medicine Humans A549 cell Matrigel Microscopy Confocal Cell Biology General Medicine medicine.disease In vitro 3. Good health Cell biology 030104 developmental biology A549 Cells Cell culture Cancer cell Taxoids Developmental Biology |
| Zdroj: | Tissue and Cell. 50:15-30 |
| ISSN: | 0040-8166 |
| Popis: | Alternative models such as three-dimensional (3D) cell cultures represent a distinct milestone towards capturing the realities of cancer biology in vitro and reduce animal experimentation in the preclinical stage of drug discovery. Significant work remains to be done to understand how substrates used in in vitro alternatives influence cancer cells phenotype and drug efficacy responses, so that to accurately link such models to specific in vivo disease scenarios. Our study describes how the morphological, mechanical and biochemical properties of adenocarcinoma (A549) cells change in response to a 3D environment and varying substrates. Confocal Laser Scanning (LSCM), He-Ion (HIM) and Atomic Force (AFM) microscopies, supported by ELISA and Western blotting, were used. These techniques enabled us to evaluate the shape, cytoskeletal organization, roughness, stiffness and biochemical signatures of cells grown within soft 3D matrices (PuraMatrix™ and Matrigel™), and to compare them to those of cells cultured on two-dimensional glass substrates. Cell cultures are also characterized for their biological response to docetaxel, a taxane-type drug used in Non-Small-Cell Lung Cancer (NSCLC) treatment. Our results offer an advanced biophysical insight into the properties and potential application of 3D cultures of A549 cells as in vitro alternatives in lung cancer research. |
| Databáze: | OpenAIRE |
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