KBMA Listeria monocytogenes is an effective vector for DC-mediated induction of antitumor immunity
| Autor: | Edward E. Lemmens, Will Luckett, Weiqun Liu, Nina Bhardwaj, Meredith L. Leong, Peter M. Lauer, Emmanuelle Godefroy, Alice W. Yewdall, Keith S. Bahjat, Thomas W. Dubensky, Dirk G. Brockstedt, Mojca Skoberne |
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| Rok vydání: | 2006 |
| Předmět: |
Antigen presentation
Priming (immunology) CD8-Positive T-Lymphocytes Major histocompatibility complex Cancer Vaccines Epitope Mice MART-1 Antigen Antigen Antigens Neoplasm Cell Line Tumor MHC class I HLA-A2 Antigen Animals Humans Melanoma Antigen Presentation Mice Inbred BALB C biology HLA-A Antigens Histocompatibility Antigens Class II General Medicine Dendritic Cells Th1 Cells Virology Listeria monocytogenes Recombinant Proteins Neoplasm Proteins Cancer research biology.protein Cytokines Cancer vaccine Research Article |
| Zdroj: | The Journal of clinical investigation. 118(12) |
| ISSN: | 0021-9738 |
| Popis: | Vaccine strategies that utilize human DCs to enhance antitumor immunity have yet to realize their full potential. Approaches that optimally target a spectrum of antigens to DCs are urgently needed. Here we report the development of a platform for loading DCs with antigen. It is based on killed but metabolically active (KBMA) recombinant Listeria monocytogenes and facilitates both antigen delivery and maturation of human DCs. Highly attenuated KBMA L. monocytogenes were engineered to express an epitope of the melanoma-associated antigen MelanA/Mart-1 that is recognized by human CD8+ T cells when presented by the MHC class I molecule HLA-A*0201. The engineered KBMA L. monocytogenes induced human DC upregulation of costimulatory molecules and secretion of pro-Th1 cytokines and type I interferons, leading to effective priming of Mart-1–specific human CD8+ T cells and lysis of patient-derived melanoma cells. KBMA L. monocytogenes expressing full-length NY-ESO-1 protein, another melanoma-associated antigen, delivered the antigen for presentation by MHC class I and class II molecules independent of the MHC haplotype of the DC donor. A mouse therapeutic tumor model was used to show that KBMA L. monocytogenes efficiently targeted APCs in vivo to induce protective antitumor responses. Together, our data demonstrate that KBMA L. monocytogenes may be a powerful platform that can both deliver recombinant antigen to DCs for presentation and provide a potent DC-maturation stimulus, making it a potential cancer vaccine candidate. |
| Databáze: | OpenAIRE |
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