Cytogenetic and DNA-fingerprint characterization of choriocarcinoma cell lines and a trophoblast/choriocarcinoma cell hybrid
| Autor: | Hitoshi Funayama, Hans-Jürgen Schulten, Bastian Gunawan, Berthold Huppertz, Ulrich Cremer, Ingke Ebeling-Stark, Hans-Georg Frank, Gabi Gaus, László Füzesi, Peter Kaufmann |
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| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
medicine.medical_specialty Biology Hybrid Cells Cell Fusion 03 medical and health sciences Chorioepithelioma 0302 clinical medicine Pregnancy Genetics medicine Tumor Cells Cultured Humans Choriocarcinoma Molecular Biology reproductive and urinary physiology In Situ Hybridization Fluorescence 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine Cell fusion medicine.diagnostic_test Cytogenetics Trophoblast Karyotype medicine.disease Molecular biology DNA Fingerprinting female genital diseases and pregnancy complications Chromosome Banding Trophoblasts medicine.anatomical_structure Cell culture Karyotyping embryonic structures Uterine Neoplasms Female Fluorescence in situ hybridization |
| Zdroj: | Cancer genetics and cytogenetics. 116(1) |
| ISSN: | 0165-4608 |
| Popis: | We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms. |
| Databáze: | OpenAIRE |
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