Polymerase Chain Reaction Approaches to Culicoides (Diptera: Ceratopogonidae) Identification
| Autor: | Mark Robertson, Walter J. Tabachnick, Julie L. Archer, Teresa J. Raich, Barry J. Beaty |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Analyte
Ceratopogonidae Molecular Sequence Data DNA Single-Stranded Polymerase Chain Reaction DNA sequencing law.invention chemistry.chemical_compound RNA Transfer law Animals Polymerase Polymerase chain reaction Genetics Orbivirus Base Sequence General Veterinary biology biology.organism_classification Molecular biology Infectious Diseases chemistry Insect Science biology.protein Parasitology DNA Specific identification |
| Zdroj: | Journal of Medical Entomology. 30:228-232 |
| ISSN: | 1938-2928 0022-2585 |
| DOI: | 10.1093/jmedent/30.1.228 |
| Popis: | Molecular genetic approaches such as polymerase chain amplification (PCR) of target genomic sequences are finding wide application in systematic and taxonomic studies of arthropods. PCR-based techniques that preclude the need for target DNA sequence information of the species of interest facilitate molecular taxonomic studies. Two such techniques, tDNA-PCR (DNA encoding tRNAs is the analyte) and RAPD-PCR (randomly amplified polymorphic DNA) were investigated for their ability to differentiate certain North American Culicoides spp. larvae and adults. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|