Specific recognition of the neuronal cell surface by an antiserum raised plasma membrane preparations of immature rat cerebellum
| Autor: | Eddi Meier, Ciaran M. Regan, Robert Balázs, G. P. Wilkin |
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| Rok vydání: | 1982 |
| Předmět: |
Cell type
Cell Biology Immunofluorescence Biochemistry Cellular and Molecular Neuroscience Antigen Cerebellum medicine Animals Cells Cultured Neurons Antiserum medicine.diagnostic_test Immune Sera Cell Membrane Membrane Proteins General Medicine Molecular biology Rats Endothelial stem cell medicine.anatomical_structure Membrane nervous system Antigens Surface Membrane fraction Immunoelectrophoresis Two-Dimensional |
| Zdroj: | Neurochemical Research. 7:1031-1043 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/bf00964884 |
| Popis: | A brain specific antiserum was prepared by immunizing rabbits with a crude membrane fraction from 8-day old rat cerebella. In immunofluorescence studies the antiserum labeled the perikarya and processes of cultured cerebellar neurones. In contrast, other cell types, encountered in cerebellar cultures including astrocytes, endothelial cells and fibroblasts, were consistently unstained. The antiserum when used in crossed immunoelectrophoresis with Triton X-100 solubilized brain extracts reacted predominantly with one antigen that could be identified as the D2 protein. |
| Databáze: | OpenAIRE |
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