PAH removal by immobilized bacterial cells-support systems using low-cost culture media for biomass production
| Autor: | Marcela Alejandra Ferrero, María Silvina Juárez Tomás, Mauricio Alessandrello, Paula Isaac, Diana L. Vullo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Calcium alginate
Pseudomonas monteilii Biotecnología del Medio Ambiente 0211 other engineering and technologies Biomass Polycyclic aromatic hydrocarbon INGENIERÍAS Y TECNOLOGÍAS 02 engineering and technology 010501 environmental sciences 01 natural sciences Microbiology Corn steep liquor LOW-COST CULTURE MEDIA Biomaterials chemistry.chemical_compound Waste Management and Disposal Bioremediación Diagnóstico Biotecnológico en Gestión Medioambiental 0105 earth and related environmental sciences Naphthalene chemistry.chemical_classification 021110 strategic defence & security studies Waste management biology food and beverages PAH Phenanthrene Pulp and paper industry biology.organism_classification chemistry Pyrene BACTERIAL CO-CULTURE IMMOBILIZATION SYSTEMS |
| Zdroj: | International Biodeterioration & Biodegradation. 120:6-14 |
| ISSN: | 0964-8305 |
| Popis: | The use of immobilized biomass for polycyclic aromatic hydrocarbon (PAH) removal from a liquid phase is a very promising technology. For this technology to be economically viable, the biomass production and immobilization should be as cost-effective as possible. The aim of this work was to produce a low-cost PAH removal system consisting of an immobilized biomass on different supports. A co-culture formed by two PAH-degrading bacteria (Pseudomonas monteilii P26 and Gordonia sp. H19, autochthonous Patagonian strains) was used. Industrial by-products such as sugar cane molasses, corn steep liquor, whey permeate and crude glycerol were used in combination to formulate different low-cost growth media. Naphthalene, phenanthrene and pyrene removal was evaluated using immobilized bacteria in polyurethane foam cubes, in sand and in calcium alginate beads. The growth of P. monteilii P26 and Gordonia sp. H19 was satisfactory in the different media assayed. The culture medium containing 1% corn steep liquor-2% molasses was selected for biomass production for subsequent immobilization. The removal of PAHs in different immobilization systems could be attributed to both the supports and the bacteria (up to 88.9% pyrene removal in 12 days); thus, immobilized cell systems for removing PAHs from an aqueous phase are proposed. Fil: Alessandrello, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina. Universidad Nacional de Tucumán; Argentina Fil: Vullo, Diana Lia. Universidad Nacional de General Sarmiento; Argentina Fil: Ferrero, Marcela Alejandra. Universidad Nacional de Tucumán; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina |
| Databáze: | OpenAIRE |
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