Development of specific enzyme-linked immunosorbent assays for multiple vitellogenins in marbled sole, Pleuronectes yokohamae
| Autor: | Naoshi Hiramatsu, Akihiko Hara, Seiichi Uno, Takashi Todo, Jiro Koyama, Haruna Amano |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Marbled meat Enzyme-Linked Immunosorbent Assay 030209 endocrinology & metabolism Body weight Andrology Vitellogenins 03 medical and health sciences 0302 clinical medicine Endocrinology Equivalent Animals Specific enzyme 030304 developmental biology Pleuronectes 0303 health sciences Estradiol biology Egg Proteins Vitellogenesis Fishes Reference Standards biology.organism_classification Polyclonal antibodies biology.protein Female Animal Science and Zoology |
| Zdroj: | General and Comparative Endocrinology. 281:67-72 |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2019.05.012 |
| Popis: | Non-competitive, enzyme-linked immunosorbent assays (ELISAs) for three distinct sole vitellogenins (VtgAa, VtgAb and VtgC) were designed using their purified lipovitellin (Lv) products and corresponding digoxigenin-labeled, anti-Lv polyclonal antibodies, primarily for employment in monitoring estrogenic pollution of the environment. The working range of the ELISAs was from 0.97 to 1,000 ng/mL for all Vtg subtypes. Each ELISA appeared to be specific to the targeted Vtg subtype. Intra- and inter-assay coefficients of variation in the developed ELISAs were lower than 10%. Three Vtg subtypes were induced in serum of immature fish by estradiol-17β (E2) injection (0.5 mg/kg body weight). All Vtg subtypes were induced one day after the injection, reaching peak levels (Lv equivalents) within three days, as follows: 39.1 ± 28.9 μg/mL (VtgAa), 57.9 ± 30.7 μg/mL (VtgAb) and 12.6 ± 4.8 μg/mL (VtgC). In wild-caught males, VtgAa, VtgAb and VtgC were detected in ranges from 0.26 to 1.21, 0.19 to 8.69, and 0.17 to 53.50 μg/mL, respectively, over various sampling periods. In vitellogenic females sampled in January, the average level of VtgAb (8,744.43 ± 733.93 μg/mL) was significantly higher than for VtgAa (150.33 ± 22.35 μg/mL) or VtgC (57.08 ± 6.00 μg/mL); thus VtgAb appeared to be the most dominant Vtg subtype. The present study entails the first report on development of subtype-specific Vtg ELISAs in marbled sole, which empowers us to detect and monitor estrogenic contamination in aquatic environments inhabited by this species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect