Differential efficacy of genetically swapping GAL4
| Autor: | Chi Jen Yang, Ya-Hui Chou, Shih-Han Lin, Ying-Jun Chen, Yuh-Tarng Chen, Cen-You Li, Hao-Hsin Chang, Hsin Ju Lin, Kai Hsiang Chang, Ting-Han Wu, Tzi-Yang Lin, Nan-Fu Liou |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Olfactory system animal structures Interneuron fungi Locus (genetics) Computational biology Biology law.invention Animals Genetically Modified Cellular and Molecular Neuroscience medicine.anatomical_structure Genetic Techniques law Transcription (biology) Genetics medicine Animals Drosophila Proteins Suppressor Drosophila Female Repressor lexA Enhancer Transcription Factors |
| Zdroj: | Journal of Neurogenetics. 33:52-63 |
| ISSN: | 1563-5260 0167-7063 |
| DOI: | 10.1080/01677063.2018.1564289 |
| Popis: | Several large or mid-scale collections of Drosophila enhancer traps have been recently created to allow for genetic swapping of GAL4 coding sequences to versatile transcription activators or suppressors such as LexA, QF, split-GAL4 (GAL4-AD and GAL4-DBD), GAL80 and QS. Yet a systematic analysis of the feasibility and reproducibility of these tools is lacking. Here we focused on InSITE GAL4 drivers that specifically label different subpopulations of olfactory neurons, particularly local interneurons (LNs), and genetically swapped the GAL4 domain for LexA, GAL80 or QF at the same locus. We found that the major utility-limiting factor for these genetic swaps is that many do not fully reproduce the original GAL4 expression patterns. Different donors exhibit distinct efficacies for reproducing original GAL4 expression patterns. The successfully swapped lines reported here will serve as valuable reagents and expand the genetic toolkits of Drosophila olfactory circuit research. |
| Databáze: | OpenAIRE |
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