Solute Carrier 11A1 Is Expressed by Innate Lymphocytes and Augments Their Activation
| Autor: | Maria E. Jerome, Deann T. Snyder, Mark A. Jutila, Jodi F. Hedges, Emily Kimmel |
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| Rok vydání: | 2013 |
| Předmět: |
Mice
129 Strain CD3 T cell Immunology Protein tyrosine phosphatase Lymphocyte Activation Article Mice chemistry.chemical_compound Antigen T-Lymphocyte Subsets Phagosome maturation medicine Animals Humans Immunology and Allergy Phosphorylation Cation Transport Proteins Mice Knockout biology Monocyte Receptors Antigen T-Cell gamma-delta Tyrosine phosphorylation Immunity Innate Up-Regulation Cell biology medicine.anatomical_structure chemistry biology.protein Tyrosine Cattle Protein Tyrosine Phosphatases Cell activation |
| Zdroj: | The Journal of Immunology. 190:4263-4273 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Solute carrier 11A1 (SLC11A1) is a divalent ion transporter formerly known as the natural resistance–associated macrophage protein (NRAMP1) and the Bcg/Lsh/Ity locus. SLC11A1 was thought to be exclusively expressed in monocyte/macrophages and to have roles in phagosome maturation and cell activation. We characterized the expression of SLC11A1 in the majority of human and bovine γδ T cells and NK cells and in human CD3+CD45RO+ T cells. Consistent with a role for iron-dependent inhibition of protein tyrosine phosphatases, SLC11A1+ lymphocytes were more prone to activation and retained tyrosine phosphorylation. Transfection of SLC11A1 into a human γδ T cell–like line rendered the cells more prone to activation. Nonadherent splenocytes from wild-type mice expressed significantly greater IFN-γ compared with cells from Sv/129 (SLC11A1−/−) mice. Our data suggest that SLC11A1 has a heretofore unknown role in activation of a large subset of innate lymphocytes that are critical sources of IFN-γ. SLC11A1+ animals have enhanced innate IFN-γ expression in response to Salmonella infection compared with SLC11A1− mice, which include commonly used inbred laboratory mice. Expression of SLC11A1 in innate lymphocytes and its role in augmenting their activation may account for inconsistencies in studies of innate lymphocytes in different animal models. |
| Databáze: | OpenAIRE |
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