Photonic crystal enhanced fluorescence immunoassay on diatom biosilica
| Autor: | Gregory L. Rorrer, Alan X. Wang, Xianming Kong, Kenneth Squire, Paul LeDuff |
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| Rok vydání: | 2018 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science General Physics and Astronomy Biosensing Techniques 02 engineering and technology 01 natural sciences General Biochemistry Genetics and Molecular Biology Fluorescence spectroscopy Microscopy General Materials Science Photonic crystal Diatoms Immunoassay Detection limit Photons business.industry Optical Imaging 010401 analytical chemistry General Engineering General Chemistry Silicon Dioxide 021001 nanoscience & nanotechnology Fluorescence Nanostructures 0104 chemical sciences Spectrometry Fluorescence Optoelectronics Photonics 0210 nano-technology business Biosensor |
| Zdroj: | Journal of Biophotonics. 11 |
| ISSN: | 1864-0648 1864-063X |
| Popis: | Fluorescence biosensing is one of the most established biosensing methods, particularly fluorescence spectroscopy and microscopy. These are two highly sensitive techniques but require high-grade electronics and optics to achieve the desired sensitivity. Efforts have been made to implement these methods using consumer grade electronics and simple optical setups for applications such as point-of-care diagnostics, but the sensitivity inherently suffers. Sensing substrates, capable of enhancing fluorescence are thus needed to achieve high sensitivity for such applications. In this paper, we demonstrate a photonic crystal-enhanced fluorescence immunoassay biosensor using diatom biosilica, which consists of silica frustules with sub-100 nm periodic pores. Utilizing the enhanced local optical field, the Purcell effect and increased surface area from the diatom photonic crystals, we create ultrasensitive immunoassay biosensors that can significantly enhance fluorescence spectroscopy as well as fluorescence imaging. Using standard antibody-antigen-labeled antibody immunoassay protocol, we experimentally achieved 100× and 10× better detection limit with fluorescence spectroscopy and fluorescence imaging respectively. The limit of detection of the mouse IgG goes down to 10-16 M (14 fg/mL) and 10-15 M (140 fg/mL) for the two respective detection modalities, virtually sensing a single mouse IgG molecule on each diatom frustule. The effectively enhanced fluorescence imaging in conjunction with the simple hot-spot counting analysis method used in this paper proves the great potential of diatom fluorescence immunoassay for point-of-care biosensing. |
| Databáze: | OpenAIRE |
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