Overexpression of miR156 in switchgrass ( Panicum virgatum L.) results in various morphological alterations and leads to improved biomass production

Autor:	Ramanjulu Sunkar, Yuhong Tang, Ji-Yi Zhang, Hui Shen, Chunxiang Fu, Jessica Matts, David G. J. Mann, Zeng-Yu Wang, Jennifer J. Wolf, Chuanen Zhou, C. Neal Stewart
Rok vydání:	2012
Předmět:	0106 biological sciences biofuel crop Apical dominance Carbohydrates Biomass Plant Science Genetically modified crops Genes Plant Panicum 01 natural sciences 7. Clean energy transgenic switchgrass 03 medical and health sciences Gene Expression Regulation Plant Bioenergy RNA Messenger Research Articles Plant Proteins 030304 developmental biology miR156 2. Zero hunger 0303 health sciences biomass microRNA biology Hydrolysis food and beverages Oryza 15. Life on land Herbaceous plant Plants Genetically Modified biology.organism_classification Energy crop MicroRNAs Panicum virgatum Solubility Agronomy Agronomy and Crop Science 010606 plant biology & botany Biotechnology
Zdroj:	Plant Biotechnology Journal
ISSN:	1467-7652 1467-7644
DOI:	10.1111/j.1467-7652.2011.00677.x
Popis:	Summary Switchgrass (Panicum virgatum L.) has been developed into a dedicated herbaceous bioenergy crop. Biomass yield is a major target trait for genetic improvement of switchgrass. microRNAs have emerged as a prominent class of gene regulatory factors that has the potential to improve complex traits such as biomass yield. A miR156b precursor was overexpressed in switchgrass. The effects of miR156 overexpression on SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes were revealed by microarray and quantitative RT-PCR analyses. Morphological alterations, biomass yield, saccharification efficiency and forage digestibility of the transgenic plants were characterized. miR156 controls apical dominance and floral transition in switchgrass by suppressing its target SPL genes. Relatively low levels of miR156 overexpression were sufficient to increase biomass yield while producing plants with normal flowering time. Moderate levels of miR156 led to improved biomass but the plants were nonflowering. These two groups of plants produced 58%‐101% more biomass yield compared with the control. However, high miR156 levels resulted in severely stunted growth. The degree of morphological alterations of the transgenic switchgrass depends on miR156 level. Compared with floral transition, a lower miR156 level is required to disrupt apical dominance. The improvement in biomass yield was mainly because of the increase in tiller number. Targeted overexpression of miR156 also improved solubilized sugar yield and forage digestibility, and offered an effective approach for transgene containment.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4d3256c605d292d1ba6a68bc868b005 https://doi.org/10.1111/j.1467-7652.2011.00677.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.