Pretreatment and Detoxification of Acid-Treated Wood Hydrolysates for Pyruvate Production by an Engineered Consortium of Escherichia coli
| Autor: | Hemshikha Rajpurohit, Mark A. Eiteman |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbial Consortia
Lignocellulosic biomass Biomass Bioengineering Borohydrides Saccharomyces cerevisiae Xylose Applied Microbiology and Biotechnology Biochemistry Lignin Hydrolysate chemistry.chemical_compound Bioreactors Pyruvic Acid Bioreactor Escherichia coli Food science Pyruvates Molecular Biology Chemistry Hydrolysis Temperature food and beverages General Medicine Microbial consortium Hydrogen-Ion Concentration Sulfuric Acids Biorefinery Wood Glucose Metabolic Engineering Fermentation Microorganisms Genetically-Modified Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. 192(1) |
| ISSN: | 1559-0291 |
| Popis: | The biorefinery concept makes use of renewable lignocellulosic biomass to produce commodities sustainably. A synthetic microbial consortium can enable the simultaneous utilization of sugars such as glucose and xylose to produce biochemicals, where each consortium member converts one sugar into the target product. In this study, woody biomass was used to generate glucose and xylose after pretreatment with 20% (w/v) sulfuric acid and 60-min reaction time. We compared several strategies for detoxification with charcoal and sodium borohydride treatments to improve the fermentability of this hydrolysate in a defined medium for the production of the growth-associated product pyruvate. In shake flask culture, the highest pyruvate yield on xylose of 0.8 g/g was found using pH 6 charcoal-treated hydrolysate. In bioreactor studies, a consortium of two engineered E. coli strains converted the mixture of glucose and xylose in batch studies to 12.8 ± 2.7 g/L pyruvate in 13 h. These results demonstrate that lignocellulosic biomass as the sole carbon source can be used to produce growth-related products after employing suitable detoxification strategies. |
| Databáze: | OpenAIRE |
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