Reducing sample consumption for serial crystallography using acoustic drop ejection
| Autor: | Robin L. Owen, A. Butryn, Danny Axford, Pierre Aller, Allen M. Orville, John H. Beale, P. T. Docker, Gabriel Leen, Bradley Davy, A. Ebrahim |
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| Přispěvatelé: | UK Government, Wellcome Trust |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Diffraction
0303 health sciences Nuclear and High Energy Physics Radiation Materials science fixed targets Drop (liquid) sample delivery 030303 biophysics Short Communications acoustic dispensing Laser law.invention Crystal 03 medical and health sciences Crystallography law serial crystallography Instrumentation Order of magnitude 030304 developmental biology |
| Zdroj: | Journal of Synchrotron Radiation |
| Popis: | A new approach for efficiently loading fixed targets for serial crystallography using acoustic dispensing is presented. Efficient sample delivery is an essential aspect of serial crystallography at both synchrotrons and X-ray free-electron lasers. Rastering fixed target chips through the X-ray beam is an efficient method for serial delivery from the perspectives of both sample consumption and beam time usage. Here, an approach for loading fixed targets using acoustic drop ejection is presented that does not compromise crystal quality, can reduce sample consumption by more than an order of magnitude and allows serial diffraction to be collected from a larger proportion of the crystals in the slurry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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