Role of dopaminergic system in core part of nucleus accumbens in hyperlocomotion and rearing induced by MK-801 in rats: A behavioral and in vivo microdialysis study

Autor: Funda Bölükbasi, Kenichi Mishima, Nobuaki Egashira, Michihiro Fujiwara, Katsunori Iwasaki, Izzettin Hatip-Al-Khatib
Jazyk: angličtina
Rok vydání: 2001
Předmět:
Male
nucleus accumbens
medicine.medical_treatment
Microdialysis
Dopamine
Motor activity
Nucleus Accumbens
3
4 dihydroxyphenylacetic acid
Stereotaxic Techniques
dizocilpine
Desipramine
dose response
Electrochemistry
rat
Saline
comparative study
Chromatography
High Pressure Liquid
MK-801
Chemistry
Dopaminergic
drug effect
article
Sympathectomy
Chemical
extracellular space
serotonin
cell level
amine
Anesthesia
medicine.symptom
medicine.drug
Serotonin
medicine.medical_specialty
animal structures
high performance liquid chromatography
animal experiment
Motor Activity
Nucleus accumbens
animal tissue
Lesion
in vivo study
Internal medicine
oxidopamine
medicine
Animals
controlled study
Rats
Wistar
Oxidopamine
Pharmacology
nonhuman
concentration (parameters)
behavior
Core nucleus accumbens (rat)
rearing
brain injury
hyperactivity
Rats
Endocrinology
dopaminergic system
nervous system
Sympatholytics
3
4-Dihydroxyphenylacetic Acid
desipramine
Dizocilpine Maleate
3
4-Dihydroxyphenylacetic Acid/metabolism
Dizocilpine Maleate/*pharmacology
Dopamine/*physiology
Excitatory Amino Acid Antagonists/*pharmacology
Extracellular Space/drug effects/metabolism
Motor Activity/drug effects/*physiology
Nucleus Accumbens/chemistry/drug effects/*physiology
Serotonin/metabolism
Extracellular Space
Excitatory Amino Acid Antagonists
Popis: We investigated modification of the MK-801 effect on motor activity and extracellular amines concentration by 6-hydroxydopamine (6-OHDA)-induced lesion of core nucleus accumbens (cACC) of rats. In vivo microdialysis-HPLC showed that the concentrations (fmol/microl) of dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and serotonin were 0.738 +/- 0.135, 155.34 +/- 41.01 and 0.334 +/- 0.024, respectively, in the cACC of intact rats. The DOPAC/DA ratio was 264.24 +/- 94.01. Unilateral lesion of the cACC with 6-OHDA (8 microg/microl) substantially reduced DA (-93%) and DOPAC (-97%) in desipramine (30 mg/kg, i.p.)-pretreated rats (6-OHDA+DMI rats) as compared to the 65% reduction rate of both amines in saline-pretreated rats (6-OHDA+saline rats). Moreover, DOPAC was reduced by 72% in 6-OHDA+DMI rats. MK-801 increased DOPAC (426-467%) and DOPAC/DA ratio (180-230%) in intact rats. On the other hand, MK-801 increased DA by 154% and 505% in 6-OHDA+saline and 6-OHDA+DMI rats, respectively. 6-OHDA reduced the effect of MK-801 on DOPAC and DOPAC/DA ratio. In the behavioral studies, MK-801 (0.01-0.3 mg/kg, i.p.) increased locomotor activity and rearing of intact rats. Bilateral 6-OHDA+DMI lesion of the cACC caused greater reduction in the effect of MK-801 (0.1 mg/kg) than that of the shell nucleus accumbens. These results suggest that increased extracellular DOPAC concentration (but not DA) and DOPAC/DA ratio in the cACC plays an important role in MK-801-hyperactivity.
Databáze: OpenAIRE
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