Localization of nucleophosmin in nuclear matrix and changes in its expression during the differentiation of human neuroblastoma induced by retinoic acid
| Autor: | San-Ying Wang, Xiu-Yan Zhang, Qi-Fu Li, Song-Lin Shi, Qing-Rong Liu, Fu-Yun Wu, Ying Liang, Guang-Jun Jing |
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| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
Retinoic acid Antineoplastic Agents Tretinoin Biology Biochemistry Proto-Oncogene Proteins c-myc Neuroblastoma chemistry.chemical_compound Nuclear Matrix-Associated Proteins Cell Line Tumor medicine Humans Nuclear Matrix Intermediate filament Molecular Biology Gel electrophoresis Nucleophosmin integumentary system Nuclear Proteins Colocalization Cell Differentiation Cell Biology medicine.disease Nuclear matrix Molecular biology chemistry Tumor Suppressor Protein p53 Proto-Oncogene Proteins c-fos |
| Zdroj: | Journal of Cellular Biochemistry. 111:67-74 |
| ISSN: | 0730-2312 |
| DOI: | 10.1002/jcb.22663 |
| Popis: | In this article, we selectively extracted the nuclear matrix and intermediate filament system of human neuroblastoma SK-N-SH cells pre- and post-treated with retinoic acid (RA). The distribution of nucleophosmin (NPM) in the nuclear matrix and its colocalization with several products of related genes were investigated. Results from two-dimensional gel electrophoresis and MALDI-TOF showed that NPM was a component of the nuclear matrix and its expression in SK-N-SH cells post-treated with RA was down-regulated. Immunofluorescent microscopy observations further showed that NPM was localized in the nuclear matrix of SK-N-SH cells, and its expression level and distribution were altered after treatment with RA. The colocalization of NPM with c-myc, c-fos, p53, and Rb in SK-N-SH cells was observed under a laser scanning confocal microscope, but the colocalization region was changed by RA. Our results prove that NPM is a nuclear matrix protein, which is localized in nuclear matrix fibers. The colocalization of NPM with its related genes and oncogenes affect the differentiation of SK-N-SH cells. The expression of NPM and its distribution in the process of cell differentiation deserve more intensive investigation. |
| Databáze: | OpenAIRE |
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