A Transcriptome Analysis of mRNAs and Long Non‐Coding RNAs in Patients with Parkinson’s Disease
| Autor: | Michele Salemi, Giuseppe Lanza, Maria Paola Mogavero, Filomena I. I. Cosentino, Eugenia Borgione, Roberta Iorio, Giovanna Maria Ventola, Giovanna Marchese, Maria Grazia Salluzzo, Maria Ravo, Raffaele Ferri |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
QH301-705.5 Long non‐coding RNAs Catalysis Inorganic Chemistry long non-coding RNAs Humans Gene Regulatory Networks Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Aged Aged 80 and over MRNAs Sequence Analysis RNA Gene Expression Profiling Organic Chemistry High-Throughput Nucleotide Sequencing Parkinson Disease RNA sequencing General Medicine Middle Aged Computer Science Applications Chemistry Case-Control Studies Parkinson’s disease Female RNA Long Noncoding Transcriptome analysis mRNAs transcriptome analysis inverse comorbidity Inverse comorbidity |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1535 International Journal of Molecular Sciences, Vol 23, Iss 1535, p 1535 (2022) |
| Popis: | Parkinson’s disease (PD) is the second most common neurodegenerative disorder. The number of cases of PD is expected to double by 2030, representing a heavy burden on the healthcare system. Clinical symptoms include the progressive loss of dopaminergic neurons in the substantia nigra of the midbrain, which leads to striatal dopamine deficiency and, subsequently, causes motor dysfunction. Certainly, the study of the transcriptome of the various RNAs plays a crucial role in the study of this neurodegenerative disease. In fact, the aim of this study was to evaluate the transcriptome in a cohort of subjects with PD compared with a control cohort. In particular we focused on mRNAs and long non-coding RNAs (lncRNA), using the Illumina NextSeq 550 DX System. Differential expression analysis revealed 716 transcripts with padj ≤ 0.05; among these, 630 were mRNA (coding protein), lncRNA, and MT_tRNA. Ingenuity pathway analysis (IPA, Qiagen) was used to perform the functional and pathway analysis. The highest statistically significant pathways were: IL-15 signaling, B cell receptor signaling, systemic lupus erythematosus in B cell signaling pathway, communication between innate and adaptive immune cells, and melatonin degradation II. Our findings further reinforce the important roles of mitochondria and lncRNA in PD and, in parallel, further support the concept of inverse comorbidity between PD and some cancers. |
| Databáze: | OpenAIRE |
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