Immunoreactivity of Tenascin-C in Dentin Matrix in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta
| Autor: | Pirjo-Liisa Lukinmaa, J. Waltimo, L. Zardi, G. Allemanni |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Adolescent Dentinogenesis imperfecta Gene Expression Epitopes 03 medical and health sciences 0302 clinical medicine stomatognathic system Dentinogenesis Imperfecta medicine Dentin Deciduous teeth Humans Tooth Deciduous Child Coloring Agents General Dentistry Dental Pulp Skin Odontoblasts biology Chemistry Tenascin C Mouth Mucosa Antibodies Monoclonal Tenascin 030206 dentistry Osteogenesis Imperfecta medicine.disease stomatognathic diseases Phenotype 030104 developmental biology medicine.anatomical_structure Odontoblast Osteogenesis imperfecta biology.protein Pulp (tooth) Female Tooth Type I collagen |
| Zdroj: | Journal of Dental Research. 75:581-587 |
| ISSN: | 1544-0591 0022-0345 |
| Popis: | Osteogenesis imperfecta (01) is a heterogeneous group of heritable connective tissue disorders, assigned to different mutations in type I collagen genes. A variety of structural abnormalities of dentin have been described in dentinogenesis imperfecta (DI) associated with OI. To clarify further the constitution of the dentin matrix in 01, we immunostained frozen and paraffin sections of deciduous teeth from four patients, each from a different family, with two monoclonal antibodies (MAbs) to the matrix glycoprotein tenascin-C (TN-C). One of the MAbs recognizes an epitope common to all TN-C isoforms (BC-4), and the other is specific for a splicing variant (BC-2). Normal teeth, oral mucosa, and skin were analyzed for comparison. Staining patterns with the two MAbs did not differ markedly. Normal dentin matrix and odontoblasts were lacking reactivity, but the pulp stained clearly. TN-C reactivity was present in the dentin matrix of all teeth obtained from two patients with different OI phenotypes and DI, and in one out of three teeth from one patient who also had DI. The reactivity was distributed in layers, but the staining patterns varied from one patient to another and from tooth to tooth. Intratubular staining seen in a tooth from the patient with clinically and histologically normal teeth was comparable with that present in normal deciduous teeth. The variation in TN-C expression suggests that, besides genetic heterogeneity, epigenetic factors could influence the composition of the dentin matrix in OI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|