Non-invasive thermal imaging of cardiac remodeling in mice
| Autor: | Olga Tepper-Shaihov, Olga Tsoref, Rafael Y. Brzezinski, Ehud Grossman, Nili Naftali-Shani, Yair Zimmer, Zehava Ovadia-Blechman, Nir Lewis, Oshrit Hoffer, Neta Rabin, La-Paz Levin-Kotler, Jonathan Leor |
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| Rok vydání: | 2019 |
| Předmět: |
Thorax
medicine.medical_specialty medicine.medical_treatment 01 natural sciences Article 010309 optics 03 medical and health sciences Fibrosis Internal medicine 0103 physical sciences Renin–angiotensin system medicine Saline 030304 developmental biology 0303 health sciences medicine.diagnostic_test business.industry Magnetic resonance imaging medicine.disease Atomic and Molecular Physics and Optics Hypertensive heart disease Blood pressure Thermography Cardiology business Biotechnology |
| Zdroj: | Biomedical Optics Express. 10:6189 |
| ISSN: | 2156-7085 |
| DOI: | 10.1364/boe.10.006189 |
| Popis: | Thermal infrared imaging has been suggested as a non-invasive alternative to monitor physiological processes and disease. However, the use of this technique to image internal organs, such as the heart, has not yet been investigated. We sought to determine the ability of our novel thermal image-processing algorithm to detect structural and functional changes in a mouse model of hypertension and cardiac remodeling. Twelve mice were randomly assigned to receive either the pro-inflammatory, hypertensive hormone angiotensin-II (2 mg/kg/day, n = 6) or saline (n = 6) infusion for 28 days. We performed weekly blood pressure measurements, together with serial trans-thoracic echocardiography studies and histopathological evaluation of the hearts. Thermal images were captured with a commercially available thermal camera, and images were processed by our novel algorithm which analyzes relative spatial temperature variation across the animal’s thorax. We assessed cardiac inflammation by measuring inflammatory cell infiltration through flow cytometry. Angiotensin infusion increased blood pressure together with cardiac hypertrophy and fibrosis. Thermal imaging at day 28 of the experiment detected an increase in the fraction of the skin heated by the heart in angiotensin-treated mice. Thermal image findings were significantly correlated to left ventricular volume and mass parameters seen on echocardiography (r = 0.8, p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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