An in vitro analysis model for investigating the staining effect of various chlorhexidine-based mouthwashes
| Autor: | Céline Bories, Xavier Struillou, Assem Soueidan, Jean-Michel Bouler, Alain Ayepa Kouadio, Zahi Badran |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
business.industry Research Chlorhexidine Dentistry 030206 dentistry CIENCIAS MÉDICAS [UNESCO] Stain Tooth discoloration Staining In vitro analysis 03 medical and health sciences 030104 developmental biology 0302 clinical medicine stomatognathic system UNESCO::CIENCIAS MÉDICAS Medicine Periodontology Food science business General Dentistry Short duration medicine.drug |
| Zdroj: | Journal of Clinical and Experimental Dentistry Kouadio, Alain Ayepa ; Struillou, Xavier ; Bories, Céline ; Bouler, Jean Michel ; Badran, Zahi ; Soueidan, Assem. An in vitro analysis model for investigating the staining effect of various chlorhexidine-based mouthwashes. En: Journal of Clinical and Experimental Dentistry, 9 3 2017: 410-416 RODERIC. Repositorio Institucional de la Universitat de Valéncia instname |
| ISSN: | 1989-5488 |
| Popis: | Background There are different mouthwashes containing chlorhexidine in different concentrations, as well as various excipients. Chlorhexidine induce stains or discoloration in teeth and mucous membranes. The aim of this work was to design a model to reproduce in vitro staining associated with the use of different mouthwashes containing chlorhexidine. Material and methods We used as substrates of natural teeth and elephant ivory slices. Different incubation baths were conducted over 21 days in culture dishes at 37°C. At the beginning of experiment before incubation (D0) and after 21 days (D21) of incubation with different mouthwashes, pictures of substrates were taken in a standardized manner and an image analysis software was used to analyse and quantify the staining under the various conditions by using the 3 main colours (Red, Green, Blue, RGB). Results The results of this work demonstrate a very good reproducibility of the protocol, and secondly, a different expression statistically significant of the primary blue colour. We suggest that for a given concentration of chlorhexidine, the staining effects may vary depending on the excipients used. Conclusions This replicable model, easy to implement over a relatively short duration, can be used for evaluation of existing mouthwashes, and to test the excipients anti discoloration proposed by manufacturers. Key words:In vitro, chlorhexidine, mouthwashes, dental stain, tooth discoloration. |
| Databáze: | OpenAIRE |
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