Nicotinic acetylcholine receptor subunit α6 associated with spinosad resistance in Rhyzopertha dominica (Coleoptera: Bostrichidae)

Autor: Mei-Er Chen, Cheng-Lung Tsai, Hang-Tang Wang
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Insecticides
DNA
Complementary
Pesticide resistance
Transcription
Genetic
Health
Toxicology and Mutagenesis
Spinosad
Receptors
Nicotinic
Real-Time Polymerase Chain Reaction
medicine.disease_cause
01 natural sciences
Insecticide Resistance
Open Reading Frames
03 medical and health sciences
Rapid amplification of cDNA ends
Complementary DNA
medicine
Animals
Amino Acids
Cloning
Molecular
Mutation
Binding Sites
biology
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
biology.organism_classification
Molecular biology
Coleoptera
Reverse transcription polymerase chain reaction
Drug Combinations
010602 entomology
Open reading frame
030104 developmental biology
Codon
Terminator
Bostrichidae
Macrolides
Agronomy and Crop Science
medicine.drug
Zdroj: Pesticide Biochemistry and Physiology. 148:68-73
ISSN: 0048-3575
Popis: The lesser grain borer, Rhyzopertha dominica, which is a primary pest of stored products, breaks up whole grains and makes them susceptible to secondary infestation by other pests. Insecticide application is the main control measure against this borer. A resistant strain of R. dominica against the insecticide, spinosad, was selected in the laboratory. The full-length cDNA of the target site of spinosad, nicotinic acetylcholine receptor subunit α6, from R. dominica (Rdα6) was cloned and analyzed using reverse transcription PCR and rapid amplification of cDNA ends. The complete 2133-bp cDNA contains the open reading frame of 1497 bp encoding a 498-amino-acid protein. There are four predicted transmembrane (TM) regions, and six extracellular ligand-binding sites at the N-terminus, upstream from the first TM in Rdα6. Three mutations have been found in the resistant strain compared with the susceptible one: (1) a 181-bp fragment truncated at the N-terminus, resulting in the appearance of a premature stop codon, (2) one missing bp at the position 997, causing a frame-shift mutation, and (3) an 87-bp fragment truncated in the TM2 region. In addition, real-time quantitative PCR was applied to detect the transcriptional expression of Rdα6 in both the susceptible and resistant strains. The results indicated that the expression of Rdα6 was significantly lower in then resistant strain than in susceptible one. In conclusion, mutation of Rdα6 may cause R. dominica resistant to spinosad due to target site insensitivity.
Databáze: OpenAIRE
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