The inhibition of restriction endonucleases due to Z-DNA in negatively supercoiled plasmid
| Autor: | Ja.M. Varschavsky, R.N. Maslova, E. A. Lesnik, Zh. A. Beschetnikova |
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| Rok vydání: | 1991 |
| Předmět: |
Molecular Sequence Data
Biophysics Cleavage (embryo) Nucleic Acid Denaturation Biochemistry Insert (molecular biology) Restriction fragment Z-DNA chemistry.chemical_compound Restriction map Plasmid Negatively supercoiled plasmid Structural Biology Genetics Escherichia coli Molecular Biology Inhibition Binding Sites biology Base Sequence DNA Superhelical Cell Biology DNA DNA Restriction Enzymes Molecular biology Restriction enzyme chemistry biology.protein Restriction endonuclease Plasmids |
| Zdroj: | FEBS letters. 280(1) |
| ISSN: | 0014-5793 |
| Popis: | Plasmid pGC20 containing the (dGC)??? insert in Sma1 recognition site has been used to study the inhibition of cleavage by different restriction endonucleases due to Z-DNA formation in (dCG)b10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to DNA conformational perturbations resulted from the Z-DNA formation. Therefore, the inhibition of DNA cleavage by a particular restriction endonucleases cannot serve as a criterion for the estimation of the length of B—Z junctions in circular supercoiled DNAs |
| Databáze: | OpenAIRE |
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