Prostaglandin E2 elevates calcium in differentiated neuroectodermal stem cells
| Autor: | Dorota A. Crawford, Hongyan Li, Christine T. Wong, Ravneet Rai-Bhogal, Jennilee M. Davidson |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Nervous system medicine.medical_specialty Growth Cones Neuronal Outgrowth chemistry.chemical_element Calcium Biology Calcium in biology Dinoprostone 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Calcium imaging Neural Stem Cells Internal medicine medicine Animals Calcium Signaling Prostaglandin E2 Growth cone Molecular Biology Cells Cultured Embryonic Stem Cells Neural Plate Cell Biology 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry lipids (amino acids peptides and proteins) Stem cell Signal transduction 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Molecular and cellular neurosciences. 74 |
| ISSN: | 1095-9327 |
| Popis: | Lipid mediator prostaglandin E2 (PGE2) is an endogenous signaling molecule that plays an important role during early development of the nervous system. Abnormalities in the PGE2 signaling pathway have been associated with neurodevelopmental disorders such as autism spectrum disorders. In this study we use ratiometric fura-2AM calcium imaging to show that higher levels of PGE2 elevate intracellular calcium levels in the cell soma and growth cones of differentiated neuroectodermal (NE-4C) stem cells. PGE2 also increased the amplitude of calcium fluctuation in the neuronal growth cones and affected the neurite extension length. In summary, our results show that PGE2 may adversely impact intracellular calcium dynamics in differentiated neuronal cells and possibly affect early development of the nervous system. |
| Databáze: | OpenAIRE |
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