Development of Fluorescent Ganglioside GD3 and GQ1b Analogs for Elucidation of Raft-Associated Interactions
| Autor: | Hidenori Tanaka, Akihiro Imamura, Miku Konishi, Naoko Komura, Yuki Suganuma, Kenichi G. N. Suzuki, Hiromune Ando, Yuya Hirose, Hideharu Ishida |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ganglioside
010405 organic chemistry Chemistry Cell Membrane Organic Chemistry Cell Raft 010402 general chemistry 01 natural sciences Fluorescence Chemical synthesis 0104 chemical sciences Membrane Microdomains medicine.anatomical_structure Membrane Gangliosides Biophysics medicine Ganglioside GD3 lipids (amino acids peptides and proteins) Lipid raft |
| Zdroj: | The Journal of Organic Chemistry. 85:15998-16013 |
| ISSN: | 1520-6904 0022-3263 |
| DOI: | 10.1021/acs.joc.0c01493 |
| Popis: | b-Series gangliosides are abundant in central nervous tissues and are involved in important nerve processes. However, their functions are complicated because of their properties of forming dynamic domains in cell plasma membranes (PMs), called lipid rafts. In this study, we aim to develop fluorescently labeled b-series gangliosides that are useful for single-molecule imaging. The chemical synthesis of fluorescent GD3 and GQ1b was achieved using sialylation and ganglioside synthetic methods previously developed by our group. Furthermore, biophysical evaluations demonstrated that synthesized fluorescent GD3 and GQ1b behaved as raft molecules on cell PMs, suggesting their applicability to the study of raft-associated interactions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|