Evaluating the diversity of Neotropical anurans using DNA barcodes
| Autor: | Ruth A. Estupiñán, Marcelo Vallinoto, Maria Paula Cruz Schneider, Stephen F. Ferrari, Maria Silvanira Barbosa, Evonnildo Costa Gonçalves |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Species complex Zoology 010603 evolutionary biology 01 natural sciences DNA barcoding Intraspecific competition Craugastor podiciferus Ranitomeya COI taxonomy 03 medical and health sciences lcsh:Zoology lcsh:QL1-991 Ecology Evolution Behavior and Systematics Scinax fuscomarginatus amphibians biology Craugastor fitzingeri Ecology Interspecific competition biology.organism_classification 030104 developmental biology identification Animal Science and Zoology Amazon basin Research Article |
| Zdroj: | ZooKeys, Vol 637, Iss, Pp 89-106 (2016) ZooKeys ZooKeys 637: 89-106 |
| ISSN: | 1313-2970 1313-2989 |
| Popis: | This study tested the effectiveness of COI barcodes for the discrimination of anuran species from the Amazon basin and other Neotropical regions. Barcodes were determined for a total of 59 species, with a further 58 species being included from GenBank. In most cases, distinguishing species using the barcodes was straightforward. Each species had a distinct COI barcode or codes, with intraspecific distances ranging from 0% to 9.9%. However, relatively high intraspecific divergence (11.4–19.4%) was observed in some species, such as Ranitomeya ventrimaculata, Craugastor fitzingeri, Hypsiboas leptolineatus, Scinax fuscomarginatus and Leptodactylus knudseni, which may reflect errors of identification or the presence of a species complex. Intraspecific distances recorded in species for which samples were obtained from GenBank (Engystomops pustulosus, Atelopus varius, Craugastor podiciferus, and Dendropsophus labialis) were greater than those between many pairs of species. Interspecific distances ranged between 11–39%. Overall, the clear differences observed between most intra- and inter-specific distances indicate that the COI barcode is an effective tool for the identification of Neotropical species in most of the cases analyzed in the present study. |
| Databáze: | OpenAIRE |
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