Determination of dimethylated arginines in human plasma by high-performance liquid chromatography
| Autor: | Lotta Uggla, Viveca Backman, Anders Pettersson |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Chromatography
Elution Extraction (chemistry) General Chemistry Arginine High-performance liquid chromatography Fluorescence Quantitative determination chemistry.chemical_compound Spectrometry Fluorescence chemistry Isomerism Human plasma Blood plasma Humans Asymmetric dimethylarginine Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 692(2) |
| ISSN: | 1387-2273 |
| Popis: | Using high-performance liquid chromatography (HPLC) with multigradient elution, N(G),N(G)-dimethyl-L-arginine (asymmetric-DMA, ADMA) and N(G),N'(G)-dimethyl-L-arginine (symmetric-DMA, SDMA) can be separated from human plasma samples. The dimethylarginine compounds in plasma, after extraction with a cation-exchange column, are converted to fluorescent derivatives with o-phthaldialdehyde (OPA) in an alkaline medium and the derivatives are separated simultaneously within 50 min on a reversed-phase column (Ultracarb 3 ODS(20)). The recoveries of ADMA and SDMA are over 80% and the method permits quantitative determination of dimethylated arginines at concentrations as low as 0.1 micromol/l in human plasma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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