Molecular signatures of tumor progression in myxoid liposarcoma identified by N-glycan mass spectrometry imaging
| Autor: | Liam A. McDonnell, Manfred Wuhrer, Michiel A. J. van de Sande, Inge H. Briaire-de Bruijn, Mar Rodríguez-Girondo, Stephanie Holst-Bernal, Bram Heijs, Marieke A. de Graaff, Judith V.M.G. Bovée |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Glycan Myxoid liposarcoma Colorectal cancer Cancer Cell Biology Biology Liposarcoma medicine.disease Pathology and Forensic Medicine carbohydrates (lipids) 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Breast cancer Tumor progression 030220 oncology & carcinogenesis medicine Cancer research biology.protein Ovarian cancer Molecular Biology |
| Zdroj: | Laboratory Investigation, 100(9), 1252-1261. NATURE PUBLISHING GROUP |
| Popis: | Myxoid liposarcoma (MLS) is the second most common subtype of liposarcoma, accounting for 6% of all sarcomas. MLS is characterized by a pathognomonic FUS-DDIT3, or rarely EWSR1-DDIT3, gene fusion. The presence of >= 5% hypercellular round cell areas is associated with a worse prognosis for the patient and is considered high grade. The prognostic significance of areas with moderately increased cellularity (intermediate) is currently unknown. Here we have applied matrix-assisted laser desorption/ionization mass spectrometry imaging to analyze the spatial distribution of N-linked glycans on an MLS microarray in order to identify molecular markers for tumor progression. Comparison of the N-glycan profiles revealed that increased relative abundances of high-mannose type glycans were associated with tumor progression. Concomitantly, an increase of the average number of mannoses on high-mannose glycans was observed. Although overall levels of complex-type glycans decreased, an increase of tri- and tetra-antennary N-glycans was observed with morphological tumor progression and increased tumor histological grade. The high abundance of tri-antennary N-glycan species was also associated with poor disease-specific survival. These findings mirror recent observations in colorectal cancer, breast cancer, ovarian cancer, and cholangiocarcinoma, and are in line with a general role of high-mannose glycans and higher-antennary complex-type glycans in cancer progression. |
| Databáze: | OpenAIRE |
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