A Comparison of E6H4 and G175-405 p16-specific Monoclonal Antibodies in Oropharyngeal Squamous Cell Carcinoma
| Autor: | Marc R. Rohrbach, Michael Schwalbe, Aparna Mahajan, Chad M. Ennis, Gregory K. Hartig |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Histology medicine.drug_class Antibodies Neoplasm Clone (cell biology) Monoclonal antibody Stain Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine McNemar's test Antibody Specificity Medicine Humans Papillomaviridae Cyclin-Dependent Kinase Inhibitor p16 Aged biology business.industry Squamous Cell Carcinoma of Head and Neck Papillomavirus Infections Antibodies Monoclonal Middle Aged biology.organism_classification Immunohistochemistry Medical Laboratory Technology Oropharyngeal Neoplasms 030104 developmental biology Oropharyngeal Carcinoma 030220 oncology & carcinogenesis biology.protein Female Antibody business |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 28(4) |
| ISSN: | 1533-4058 |
| Popis: | The purpose of this investigation is to directly compare G175-405 and E6H4 p16-specific antibodies as immunomarkers of HPV-driven oropharyngeal carcinoma. The investigators designed a retrospective analysis using specimens from an archived tissue bank with known in situ hybridization and polymerase chain reaction status for HPV DNA. Fifty randomly selected oropharyngeal specimens were evaluated with both the G175-405 and E6H4 p16-specific monoclonal antibodies. Two pathologists, blinded to the HPV-specific testing status, evaluated p16 positivity for both antibody clones. Interrater agreement was determined using a Cohen κ coefficient. Sensitivity and specificity values were calculated using a standard 2×2 contingency table, then compared using McNemar test. Interrater agreement for interpretation of p16 expression was 92% (κ=0.84) for the G175-405 clone and 100% for the E6H4 clone (κ=1.0). The G175-405 stain had a sensitivity of 0.917 and specificity of 0.846. The E6H4 stain had a sensitivity of 1.000 and specificity of 0.769. Using McNemar test, there were no significant differences found for sensitivity (P=0.480) or specificity (P=0.480) values. The results of this study suggest that though both G175-405 and E6H4 antibody stains are statistically comparable immunomarkers for HPV-driven oropharyngeal carcinoma, the E6H4 clone offers improved interobserver reliability. |
| Databáze: | OpenAIRE |
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