Role of trehalose biosynthesis in environmental survival and virulence of Salmonella enterica serovar typhimurium
| Autor: | Angela M. Howells, Graeme Frith, Arcadio Garcı́a de Castro, Alan Tunnacliffe, Kam Dhaliwal, Richard W. Titball, Helen L. Bullifent, Kate F. Griffin |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Salmonella typhimurium Hot Temperature Operon Mutant Virulence Polymerase Chain Reaction Microbiology Mice chemistry.chemical_compound Osmotic Pressure Animals Cloning Molecular Molecular Biology Gene Mice Inbred BALB C Osmotic concentration biology Trehalose General Medicine biology.organism_classification Phosphoric Monoester Hydrolases Mutagenesis Insertional chemistry Glucosyltransferases Salmonella enterica Female Bacteria |
| Zdroj: | Research in Microbiology. 153:281-287 |
| ISSN: | 0923-2508 |
| Popis: | The otsA and otsB genes, encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase respectively, have been isolated from Salmonella enterica serovar typhimurium and nucleotide-sequenced. Induction of trehalose biosynthesis by exposure of bacteria to high osmotic strength resulted in the intracellular accumulation of trehalose. An otsA mutant of S. enterica serovar typhimurium was more susceptible to killing by heat, and grew poorly under conditions of high osmolarity. The wild-type and otsA mutant strains showed similar abilities to colonise spleen tissues after oral dosing of mice. These findings suggest that the otsBA gene products play a role in environmental survival, but not in virulence, of S. enterica serovar typhimurium. |
| Databáze: | OpenAIRE |
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