Genetic variation at the uncoupling protein 1, 2 and 3 loci and the response to long-term overfeeding
| Autor: | Yvon C. Chagnon, G. Sun, Claude Bouchard, André J. Tremblay, Olavi Ukkola |
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| Rok vydání: | 2024 |
| Předmět: |
Adult
Male medicine.medical_specialty Thyroid Hormones Genetic Linkage Medicine (miscellaneous) Clinical nutrition Biology Polymerase Chain Reaction Ion Channels Mitochondrial Proteins Thyroid-stimulating hormone Internal medicine medicine Uncoupling protein Humans Uncoupling Protein 3 Uncoupling Protein 2 Obesity Uncoupling Protein 1 UCP3 Nutrition and Dietetics Uncoupling Agents Genetic Variation Membrane Proteins Membrane Transport Proteins Proteins Twins Monozygotic Thermogenin Respiratory quotient Endocrinology Basal metabolic rate Body Composition Basal Metabolism medicine.symptom Carrier Proteins Energy Intake Energy Metabolism Weight gain Polymorphism Restriction Fragment Length |
| Zdroj: | European Journal of Clinical Nutrition. 55:1008-1015 |
| ISSN: | 0954-3007 |
| Popis: | Objective: To evaluate the effects of uncoupling protein (UCP) 1, UCP2 and UCP3 gene variants on body composition and metabolic changes in response to chronic overfeeding and the recovery after the period of overfeeding. Subjects and design: Twenty-four normal weight men (21±2 y), who constituted 12 pairs of identical twins, ate a 4.2 MJ/day energy surplus, 6 days a week, during a period of 100 days. The subjects were asked to return to the laboratory for testing at 4 months and for a final examination 5 y after completion of the overfeeding protocol. Methods: Resting metabolic rate (RMR) measurements were performed before and after overfeeding. A 4.2 MJ test meal was consumed, after which calorimetric measurements were continued for 240 min. Total body fat was assessed by hydrodensitometry and total subcutaneous fat by the sum of eight skinfolds. Polymorphisms were typed by PCR and PCR-RFLP-techniques. Thyroid stimulating hormone (TSH) concentrations after a thyrotropin releasing hormone (TRH) injection were measured by radioimmunoassay (RIA). Results: The changes in body weight and adiposity were not different between UCP1 Bcl I, UCP2 alanine to valine (A55V), UCP2 insertion/deletion (I/D) or UCP3 Rsa I genotypes. However, the recovery from overfeeding was worse among G-allele carriers of the UCP1 Bcl I, I allele non-carriers of the UCP2 I/D, AV heterozygote subjects of the UCP2 A55V and CC subjects of the UCP3 Rsa I polymorphisms. RMR was lower both before (P=0.01) and after (P=0.001) overfeeding in subjects with the CC genotype of the UCP3 Rsa I polymorphism. Moreover, after overfeeding, the UCP2 A55V heterozygote and UCP3 Rsa I CC homozygote subjects had significantly higher respiratory quotient (RQ) values at rest (P |
| Databáze: | OpenAIRE |
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