Comparison of human isogeneic Wharton’s jelly MSCs and iPSC-derived MSCs reveals differentiation-dependent metabolic responses to IFNG stimulation
| Autor: | Francesco Dazzi, Athanasios Mantalaris, Liani Devito, Carl Hobbs, Dusko Ilic, Yacoub Khalaf, Antonio Galleu, Aleksandra Cvoro, Michail E. Klontzas, Marisa Simon |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Stromal cell Cell Plasticity Induced Pluripotent Stem Cells Immunology Biology Article Umbilical Cord Cell therapy Transcriptome Interferon-gamma 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Wharton's jelly Humans Cellular Reprogramming Techniques lcsh:QH573-671 Induced pluripotent stem cell Cells Cultured Cell Proliferation Cell growth lcsh:Cytology Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Cellular Reprogramming Cell biology 030104 developmental biology 030220 oncology & carcinogenesis Metabolome Female |
| Zdroj: | Cell Death and Disease, Vol 10, Iss 4, Pp 1-13 (2019) Cell Death & Disease |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-019-1498-0 |
| Popis: | Variability among donors, non-standardized methods for isolation, and characterization contribute to mesenchymal stem/stromal cell (MSC) heterogeneity. Induced pluripotent stem cell (iPSCs)-derived MSCs would circumvent many of current issues and enable large-scale production of standardized cellular therapy. To explore differences between native MSCs (nMSCs) and iPSC-derived MSCs (iMSCs), we developed isogeneic lines from Wharton’s jelly (WJ) from the umbilical cords of two donors (#12 and #13) under xeno-free conditions. Next, we reprogrammed them into iPSCs (iPSC12 and iPSC13) and subsequently differentiated them back into iMSCs (iMSC12 and iMSC13) using two different protocols, which we named ARG and TEX. We assessed their differentiation capability, transcriptome, immunomodulatory potential, and interferon-γ (IFNG)-induced changes in metabolome. Our data demonstrated that although both differentiation protocols yield iMSCs similar to their parental nMSCs, there are substantial differences. The ARG protocol resulted in iMSCs with a strong immunomodulatory potential and lower plasticity and proliferation rate, whereas the TEX protocol raised iMSCs with a higher proliferation rate, better differentiation potential, though weak immunomodulatory response. Our data suggest that, following a careful selection and screening of donors, nMSCs from umbilical’s cord WJ can be easily reprogrammed into iPSCs, providing an unlimited source of material for differentiation into iMSCs. However, the differentiation protocol should be chosen depending on their clinical use. |
| Databáze: | OpenAIRE |
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