Protein MRI contrast agent with unprecedented metal selectivity and sensitivity for liver cancer imaging
| Autor: | Fan Pu, Shenghui Xue, Kendra Hubbard, Mani Salarian, Hans E. Grossniklaus, Hua Yang, Robert C. Long, Jingjuan Qiao, Xiaoping Philip Hu, Jie Jiang, Jenny J. Yang, Khan Hekmatyar, Zhi-Ren Liu, Robert G. Bryant, Jason Langley |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular Pathology medicine.medical_specialty MRI contrast agent Melanoma Experimental Contrast Media Gadolinium Biology Protein Engineering Metastasis Mice Liver Neoplasms Experimental Pharmacokinetics Limit of Detection In vivo Cell Line Tumor medicine Animals Multidisciplinary Protein Stability Biological Sciences medicine.disease Magnetic Resonance Imaging Recombinant Proteins Mice Inbred C57BL Parvalbumins Toxicity Drug delivery Cancer research Female Liver cancer Selectivity |
| Zdroj: | Proceedings of the National Academy of Sciences. 112:6607-6612 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1423021112 |
| Popis: | Significance Primary and metastatic liver cancers that are associated with high mortality rates and poor treatment responses are only diagnosed at late stages, due to the lack of highly sensitive contrast agents and robust imaging methodologies. We have developed a protein MRI contrast agent (ProCA32) by engineering high-affinity Gd 3+ -binding pockets in rat and human α-parvalbumin. ProCA32 can function as both a T 1 - and T 2 -weighted contrast agent, which enables noninvasive detection of early-stage micrometastatic liver tumors with sizes as small as 0.24 mm using T 1 - and T 2 -weighted or T 2 /T 1 ratio MRI. Our protein-based MRI contrast agents and imaging methodology are expected to provide robust results for the early detection of liver cancer as well as other liver diseases. |
| Databáze: | OpenAIRE |
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