Physical Principles of Membrane Shape Regulation by the Glycocalyx
| Autor: | Hao Pan, Matthew J. Paszek, John P. O’Donnell, Michael J. Hollander, Carolyn R. Shurer, Carolyn R. Bertozzi, Thais A. Enoki, Jin Su, Kayvon Pedram, W. E. Moerner, Leonhard Möckl, Jade M. Noble, LaDeidra Monet Roberts, Gerald W. Feigenson, Heidi L. Reesink, Jay G. Gandhi, Rose Yin, Marshall J. Colville, Joe Chin-Hun Kuo, Lena F. Kourkoutis |
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| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Membrane Glycoproteins Microvesicle Mucins Biology Polymer brush Glycocalyx General Biochemistry Genetics and Molecular Biology Cell Line Extracellular Matrix Extracellular matrix 03 medical and health sciences 0302 clinical medicine Membrane Membrane curvature Biophysics Animals Humans Secretion Horses Cytoskeleton Cell Shape 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Cell. 177(7) |
| ISSN: | 1097-4172 |
| Popis: | Cells bend their plasma membranes into highly curved forms to interact with the local environment, but how shape generation is regulated is not fully resolved. Here, we report a synergy between shape-generating processes in the cell interior and the external organization and composition of the cell-surface glycocalyx. Mucin biopolymers and long-chain polysaccharides within the glycocalyx can generate entropic forces that favor or disfavor the projection of spherical and finger-like extensions from the cell surface. A polymer brush model of the glycocalyx successfully predicts the effects of polymer size and cell-surface density on membrane morphologies. Specific glycocalyx compositions can also induce plasma membrane instabilities to generate more exotic undulating and pearled membrane structures and drive secretion of extracellular vesicles. Together, our results suggest a fundamental role for the glycocalyx in regulating curved membrane features that serve in communication between cells and with the extracellular matrix. |
| Databáze: | OpenAIRE |
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