CD4 + Th1-cells Predominate in Low-grade B-Cell Lymphoma of Gastric Mucosa-associated Lymphoid Tissue (MALT type)
| Autor: | Norbert Lügering, Torsten Kucharzik, M. Tiemann, M. Kraft, Hans-Gerd Pauels, Wolfram Domschke, Alexander Steinbüchel, Susanne Riedel |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Pathology medicine.medical_specialty CD4-CD8 Ratio Cell Separation Interferon-gamma Stomach Neoplasms immune system diseases hemic and lymphatic diseases Submucosa medicine Gastric mucosa Humans Aged biology business.industry Gastroenterology MALT lymphoma Lymphoma B-Cell Marginal Zone Middle Aged Helicobacter pylori Flow Cytometry medicine.disease biology.organism_classification Lymphoma medicine.anatomical_structure Lymphatic system Female Gastritis medicine.symptom business CD8 |
| Zdroj: | Scandinavian Journal of Gastroenterology. 36:1198-1203 |
| ISSN: | 1502-7708 0036-5521 |
| DOI: | 10.1080/00365520152584842 |
| Popis: | Little is known about the function of T cells in the inflammatory infiltrate in Helicobacter pylori-associated gastritis and B-cell lymphoma of mucosa-associated lymphoid tissue (MALT type). Previous studies have proposed a dominant Th1-type response in low-grade MALT lymphoma consistent with the Th1 response observed in H. pylori-associated gastritis.We performed a novel flow cytometric approach in which CD3 panning for enrichment and activation of small numbers of T cells and intracellular cytokine analysis were combined to selectively characterize the cytokine profile of T cells (IFN-gamma for Th1) derived from the gastric mucosa of 23 patients with low-grade MALT lymphoma stage IEI1 (lymphoma infiltration of mucosa/submucosa sparing the muscularis). Endosonography was performed in each case to control the depth of lymphoma infiltration. For comparison, 19 patients with H. pylori-positive gastritis were also analysed.There was a CD4/CD8 ratio of 4 in patients with MALT lymphoma and of 2 in chronic gastritis. The proportion of IFN-gamma producing cells within the CD4-positive T-cell population in MALT lymphoma was 22%; in chronic gastritis it was 13% while no such difference could be encountered in CD8-positive T cells.The data point towards a dominant intratumoral IFN-gamma dominated T-cell response associated with early low-grade MALT lymphoma. A polarized IFN-gamma dominated Th1-type response may either contribute to the inability of the immune system to eradicate H. pylori infection, thereby promoting the activation status of the lymphocytic infiltrate in low-grade MALT lymphoma, or may mirror a concomitant tumor-specific T-cell response accompanying early stages of tumor progression. |
| Databáze: | OpenAIRE |
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