Real-time deformability cytometry: on-the-fly cell mechanical phenotyping
| Autor: | Ulrich F. Keyser, Christoph Herold, Stefano Pagliara, Alexander Mietke, Stefan Golfier, Jörg Mansfeld, Angela Jacobi, Elisabeth Fischer-Friedrich, Nicole Töpfner, Andrew Ekpenyong, Philipp Rosendahl, Manja Wobus, Daniel Klaue, Oliver Otto, Jochen Guck, Salvatore Girardo |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cytochalasin D
On the fly Cellular differentiation Cell Antigens CD34 HL-60 Cells Biology Biochemistry law.invention Flow cytometry law medicine Humans Cell Lineage Cell Shape Molecular Biology Cytoskeleton Whole blood medicine.diagnostic_test Cell Cycle Cell Differentiation Equipment Design Cell Biology Microfluidic Analytical Techniques Lab-on-a-chip Flow Cytometry Hematopoietic Stem Cells Living matter Cell biology medicine.anatomical_structure Cytometry Biotechnology Biomedical engineering |
| Zdroj: | Nature Methods |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.3281 |
| Popis: | We introduce real-time deformability cytometry (RT-DC) for continuous cell mechanical characterization of large populations (100,000 cells) with analysis rates greater than 100 cells/s. RT-DC is sensitive to cytoskeletal alterations and can distinguish cell-cycle phases, track stem cell differentiation into distinct lineages and identify cell populations in whole blood by their mechanical fingerprints. This technique adds a new marker-free dimension to flow cytometry with diverse applications in biology, biotechnology and medicine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|