Glycosyltransferase and sulfotransferase gene expression profiles in human monocytes, dendritic cells and macrophages

Autor: Marie-Ange Krzewinski-Recchi, François Trottein, Steven R. Head, Philippe Gosset, Philippe Delannoy, Suzanna Lee, Catherine Vendeville, Stoyan Ivanov, Christophe Paget, Lana Schaffer, Christelle Faveeuw, Aurélie Cazet, Sophie Groux-Degroote
Přispěvatelé: Schistosomiase, paludisme et inflammation, Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille, Droit et Santé, The SCRIPPS Research Institute (SCRIPPS), University of California [Los Angeles] (UCLA), University of California-University of California, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Biomolécules et inflammation pulmonaire, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), The Scripps Research Institute [La Jolla, San Diego], Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Glycoconjugate Journal
Glycoconjugate Journal, Springer Verlag, 2009, 26 (9), pp.1259-1274. ⟨10.1007/s10719-009-9244-y⟩
Glycoconjugate Journal, 2009, 26 (9), pp.1259-1274. ⟨10.1007/s10719-009-9244-y⟩
ISSN: 0282-0080
1573-4986
DOI: 10.1007/s10719-009-9244-y⟩
Popis: International audience; Using a focused glycan-gene microarray, we compared the glycosyltransferase (GT) and sulfotransferase gene expression profiles of human monocytes, dendritic cells (DCs) and macrophages (Mvarphis), isolated or differentiated from the same donors. Microarray analysis indicated that monocytes express transcripts for a full set of enzymes involved in the biosynthesis of multi-multiantennary branched N-glycans, potentially elongated by poly-N-acetyl-lactosamine chains, and of mucin-type Core 1 and Core 2 sialylated O-glycans. Monocytes also express genes involved in the biosynthesis and modification of glycosaminoglycans, but display a limited expression of GTs implicated in glycolipid synthesis. Among genes expressed in monocytes (90 out of 175), one third is significantly modulated in DCs and Mvarphi respectively, most of them being increased in both cell types relative to monocytes. These changes might potentially enforce the capacity of differentiated cells to synthesize branched N-glycans and mucin-type O-glycans and to remodel cell surface proteoglycans. Stimulation of DCs and Mvarphis with lipopolysaccharide caused a general decrease in gene expression, mainly affecting genes found to be positively modulated during the differentiation steps. Interestingly, although a similar set of enzymes are modulated in the same direction in mature DCs and Mvarphis, cell specific genes are also differentially regulated during maturation, a phenomenon that may sustain functional specificities. Validation of this analysis was provided by quantitative real-time PCR and flow cytometry of cell surface glycan antigens. Collectively, this study implies an important modification of the pattern of glycosylation in DCs and Mvarphis undergoing differentiation and maturation with potential biological consequences.
Databáze: OpenAIRE
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