Vitamin E does not regress hypercholesterolemia-induced oxidative stress in heart
Autor: | A. Mabood Qureshi, Erick D. McNair, Kailash Prasad, Gudrun Caspar-Bell |
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Rok vydání: | 2014 |
Předmět: |
medicine.medical_specialty
Antioxidant Clinical chemistry medicine.medical_treatment Hypercholesterolemia Clinical Biochemistry medicine.disease_cause chemistry.chemical_compound Malondialdehyde Internal medicine High cholesterol diet medicine Animals Vitamin E Regular diet Molecular Biology Triglycerides Serum cholesterol Myocardium Cell Biology General Medicine Oxidative Stress Endocrinology chemistry Luminescent Measurements Rabbits Oxidative stress |
Zdroj: | Molecular and Cellular Biochemistry. 391:211-216 |
ISSN: | 1573-4919 0300-8177 |
Popis: | Vitamin E suppresses the hypercholesterolemia-induced cardiac oxidative stress. The objectives were to investigate: if vitamin E regresses the hypercholesterolemia-induced oxidative stress in hearts and if regression is associated with decreases in the antioxidant reserve. The rabbits were assigned to 4 groups: I, regular diet (2-months); II, 0.25% cholesterol diet (2-months); III, 0.25% cholesterol diet (2-months) followed by regular diet (2-months); and IV, 0.25% cholesterol diet (2-months) followed by regular diet with vitamin E (2-months). Blood samples were collected before and at the end of protocol for the measurement of total cholesterol (TC). Hearts were removed at the end of the protocol under anesthesia for the assessment of oxidative stress parameters, malondialdehyde (MDA), and tissue chemiluminescent (CL) activity. High cholesterol diet increased the serum levels of TC, and regular diet with or without vitamin E reduced the TC levels to a similar extent. The MDA content of the heart in groups I, II, III, and IV were 0.074 ± 0.015, 0.234 ± 0.016, 0.183 ± 0.028 and 0.169 ± 0.016 nmol/mg protein, respectively. Regular diet following high cholesterol diet reduced the MDA levels (0.234 ± 0.016 vs. 0.183 ± 0.028 nmol/mg protein but vitamin E did not reduce the MDA levels. The cardiac-CL activities were similar in groups' I, II, and III (30.11 ± 0.7 × 10(6), 32.9 ± 1.43, and 37.92 ± 8.35 × 10(6) RLU/mg protein). The activity decreased in group IV, suggesting that vitamin E increased the antioxidant reserve while lowering serum cholesterol did not increase antioxidant reserve. In conclusion, hypercholesterolemia increases cardiac oxidative stress and regular diet regresses hypercholesterolemia-induced oxidative stress but vitamin E does not further regress hypercholesterolemia-induced cardiac oxidative stress. Vitamin E reduces oxidative stress in the heart tissue in spite of a decrease in CL activity (increase in antioxidant reserve). |
Databáze: | OpenAIRE |
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