Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum
| Autor: | Yanru Hu, Liang Shi, Mingwen Zhao, Jiale Xia, Ang Ren, Shishan Hu, Lingdan Lian, Jing Zhu, Wenzhao Xu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Reishi beta-Glucans Osmotic shock Promoter Biology Microbiology Adaptation Physiological Cell biology Cell wall Fungal Proteins Oxidative Stress chemistry Cell Wall Glucosyltransferases Gene Expression Regulation Fungal Gene expression Secondary metabolism Transcription factor Gene Glucan Transcription Factors |
| Zdroj: | Microbiological research. 230 |
| ISSN: | 1618-0623 |
| Popis: | The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear. In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed. In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains. In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains. Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression. The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes. |
| Databáze: | OpenAIRE |
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