Mini-P-gp and P-gp Co-Expression in Brown Trout Erythrocytes: A Prospective Blood Biomarker of Aquatic Pollution
| Autor: | François Desmolles, Bruno Combourieu, Mahchid Bamdad, Christian Amblard, Frédérique Penault-Llorca, Emeline Valton |
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| Přispěvatelé: | Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Synthèse et étude de systèmes à intêret biologique (SEESIB), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Centre Jean Perrin [Clermont-Ferrand] (UNICANCER/CJP), UNICANCER, Laboratoire Biochimie Médicale (UMR 384), Institut National de la Santé et de la Recherche Médicale (INSERM), BRICHEUX, Genevieve, Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2015 |
| Předmět: |
Clinical Biochemistry
[SDV.MP.PRO] Life Sciences [q-bio]/Microbiology and Parasitology/Protistology 010501 environmental sciences [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology 01 natural sciences Article Microbiology 03 medical and health sciences chemistry.chemical_compound Brown trout brown trout [SDV.EE.SANT] Life Sciences [q-bio]/Ecology environment/Health ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0105 earth and related environmental sciences [SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/Health Pollutant lcsh:R5-920 0303 health sciences biology river pollution Pesticide biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology In vitro [SDV.TOX] Life Sciences [q-bio]/Toxicology Multiple drug resistance Biomarker Trout chemistry [SDV.TOX]Life Sciences [q-bio]/Toxicology Environmental chemistry erythrocytes mini-P-gp P-gp [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology blood biomarker lcsh:Medicine (General) Xenobiotic |
| Zdroj: | Diagnostics Volume 5 Issue 1 Pages 10-26 Diagnostics, Vol 5, Iss 1, Pp 10-26 (2015) Diagnostics, MDPI, 2015, 5 (1), pp.10-26 Diagnostics, 2015, 5 (1), pp.10-26 |
| ISSN: | 2075-4418 |
| DOI: | 10.3390/diagnostics5010010 |
| Popis: | In aquatic organisms, such as fish, blood is continually exposed to aquatic contaminants. Multidrug Resistance (MDR) proteins are ubiquitous detoxification membrane pumps, which recognize various xenobiotics. Moreover, their expression is induced by a large class of drugs and pollutants. We have highlighted the co-expression of a mini P-gp of 75 kDa and a P-gp of 140 kDa in the primary culture of brown trout erythrocytes and in the erythrocytes of wild brown trout collected from three rivers in the Auvergne region of France. In vitro experiments showed that benzo[a]pyrene, a highly toxic pollutant model, induced the co-expression of mini-P-gp and P-gp in trout erythrocytes in a dose-dependent manner and relay type response. Similarly, in the erythrocytes of wild brown trout collected from rivers contaminated by a mixture of PAH and other multi-residues of pesticides, mini-P-gp and P-gp were able to modulate their expression, according to the nature of the pollutants. The differential and complementary responses of mini-P-gp and P-gp in trout erythrocytes suggest the existence in blood cells of a real protective network against xenobiotics/drugs. This property could be exploited to develop a blood biomarker of river pollution. |
| Databáze: | OpenAIRE |
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