Bioelectrical Interfaces with Cortical Spheroids in Three-Dimensions
| Autor: | Yingqiao Wang, Devora Cohen-Karni, Esther Bedoyan, Tzahi Cohen-Karni, Raghav Garg, Maysamreza Chamanzar, Anna Kalmykov, Jay W. Reddy, Sahil K. Rastogi |
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| Rok vydání: | 2020 |
| Předmět: |
Neurons
Future studies Chemistry Drug discovery 0206 medical engineering Biomedical Engineering Glutamate receptor Spheroid Biosensing Techniques 02 engineering and technology Multielectrode array Nervous System 020601 biomedical engineering Excitatory neurotransmitter 03 medical and health sciences Cellular and Molecular Neuroscience Microelectrode Electrophysiology 0302 clinical medicine Spike sorting Spheroids Cellular Humans Microelectrodes Neuroscience 030217 neurology & neurosurgery |
| DOI: | 10.1101/2020.11.29.401638 |
| Popis: | Objective. Three-dimensional (3D) neuronal spheroid culture serves as a powerful model system for the investigation of neurological disorders and drug discovery. The success of such a model system requires techniques that enable high-resolution functional readout across the entire spheroid. Conventional microelectrode arrays and implantable neural probes cannot monitor the electrophysiology (ephys) activity across the entire native 3D geometry of the cellular construct. Approach. Here, we demonstrate a 3D self-rolled biosensor array (3D-SR-BA) integrated with a 3D cortical spheroid culture for simultaneous in vitro ephys recording, functional Ca2+ imaging, while monitoring the effect of drugs. We have also developed a signal processing pipeline to detect neural firings with high spatiotemporal resolution from the ephys recordings based on established spike sorting methods. Main results. The 3D-SR-BAs cortical spheroid interface provides a stable, high sensitivity recording of neural action potentials (µV peak-to-peak amplitude). The 3D-SR-BA is demonstrated as a potential drug screening platform through the investigation of the neural response to the excitatory neurotransmitter glutamate. Upon addition of glutamate, the neural firing rates increased notably corresponding well with the functional Ca2+ imaging. Significance. Our entire system, including the 3D-SR-BA integrated with neuronal spheroid culture, enables simultaneous ephys recording and functional Ca2+ imaging with high spatiotemporal resolution in conjunction with chemical stimulation. We demonstrate a powerful toolset for future studies of tissue development, disease progression, and drug testing and screening, especially when combined with native spheroid cultures directly extracted from humans. |
| Databáze: | OpenAIRE |
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