Olivocochlear innervation of inner and outer hair cells during postnatal maturation: an immunocytochemical study
| Autor: | Hugh D. Moulding, Daniel Zee, Dwayne D. Simmons |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Calcitonin Gene-Related Peptide Efferent Population Synaptogenesis Nerve Tissue Proteins Olivary Nucleus Biology Efferent Pathways Synaptic vesicle Neurons Efferent Developmental Neuroscience Cricetinae Internal medicine otorhinolaryngologic diseases medicine Animals Inner ear education Cochlea education.field_of_study Hair Cells Auditory Inner Mesocricetus Cochlear nerve Immunohistochemistry Hair Cells Auditory Outer Microscopy Electron Endocrinology medicine.anatomical_structure nervous system Synaptophysin biology.protein Synaptic Vesicles sense organs Developmental Biology |
| Zdroj: | Developmental Brain Research. 95:213-226 |
| ISSN: | 0165-3806 |
| DOI: | 10.1016/0165-3806(96)00084-3 |
| Popis: | Changes in the expression of several neurochemical markers associated with either axonal growth (GAP-43), synaptic vesicles (synaptophysin), or the cholinergic population of lateral olivocochlear (OC) efferents were investigated in the postnatal cochlea of hamsters. Growth-associated protein was expressed in the neonatal cochlea but not in the adult; immunoreactivity was found below inner hair cells (IHCs) from postnatal day (P) 2 through P14 and below outer hair cells (OHCs) from P5 to P14. In contrast, synaptophysin was expressed in both the neonate and adult cochlea; immunoreactivity was found below IHCs around P4 and below OHCs at P5. Both GAP-43 and synaptophysin immunoreactivities occurred first below IHCs in basal regions of the cochlea. Efferent fibers containing calcitonin gene-related peptide (CGRP) immunoreactivity were identified as early as P4 within the cochlear nerve but were not observed underneath IHCs until P7. Similar to GAP-43 and synaptophysin immunoreactivity, CGRP expression followed a basal to apical gradient; however, expression below OHCs appeared restricted to apical regions. These data raise the possibility that efferents expressing growth proteins and efferents expressing synaptic vesicle proteins co-exist during the first postnatal week. Furthermore, it is hypothesized that CGRP-containing lateral OC neurons form part of a later, secondary innervation to the cochlea. |
| Databáze: | OpenAIRE |
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