Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) hybrid nanomaterials as immunoassay label for low-density lipoprotein detection

Autor: Akarapong Prakobkij, Purim Jarujamrus, Suticha Chunta, Runglawan Chawengkirttikul, Tinnagon Keawin, Nutthaporn Malahom, Suparb Tamuang, Maliwan Amatathongchai, Daniel Citterio
Rok vydání: 2021
Předmět:
Zdroj: Mikrochimica acta. 189(2)
ISSN: 1436-5073
Popis: Nitrogen-doped carbon dots/Ni-MnFe-layered double hydroxides (N-CDs/Ni-MnFe-LDHs) are demonstrated as superior peroxidase mimic antibody labels alternative to horseradish peroxidase (HRP) in an immunoassay, potentially overcoming some of the inherent disadvantages of HRP and other enzyme mimicking nanomaterials. They revealed efficient peroxidase-like activity and catalyzed the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to form the intense blue product (at 620 nm) in the presence of hydrogen peroxide (H
Databáze: OpenAIRE