Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes
| Autor: | Fabian Bartlog, Sönke Harder, Stefanie Wienkoop, Sabine Lüthje, Anne Hofmann |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteome plasma membrane 01 natural sciences Plant Roots lcsh:Chemistry Cell Wall Gene Expression Regulation Plant Tandem Mass Spectrometry Gene expression lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification Phenylpropanoid biology Chemistry food and beverages General Medicine Cell Hypoxia Computer Science Applications Isoenzymes Biochemistry Peroxidases class III peroxidases aerenchyma Oxidation-Reduction maize roots Peroxidase Protein Binding respiratory burst oxidase homolog Zea mays L Zea mays Catalysis Article Aerenchyma Aerenchyma formation Inorganic Chemistry Cell wall 03 medical and health sciences Suberin Physical and Theoretical Chemistry Molecular Biology Reactive oxygen species hypoxia fungi Organic Chemistry Cell Membrane NADPH Oxidases cell wall remodeling 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 biology.protein Reactive Oxygen Species 010606 plant biology & botany Chromatography Liquid |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 22 International Journal of Molecular Sciences, Vol 21, Iss 8872, p 8872 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize (Zea mays L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC&ndash MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of ZmPrx03, ZmPrx24, ZmPrx81, and ZmPr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of ZmPrx03 and ZmPrx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections. |
| Databáze: | OpenAIRE |
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