Identification and metagenetic characterisation of Listeria monocytogenes-harbouring communities present in food-related industrial environments
| Autor: | Pedro Rodríguez-López, Marta López Cabo, Angel S. Comesaña, Marta Bernárdez, Juan José Rodríguez-Herrera |
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| Přispěvatelé: | Ministerio de Economía, Industria y Competitividad (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Food industry
Lactococcus medicine.disease_cause 01 natural sciences Actinobacteria Food safety 0404 agricultural biotechnology Listeria monocytogenes Lactobacillus medicine High throughput sequencing Food science Survey biology business.industry 010401 analytical chemistry 04 agricultural and veterinary sciences biology.organism_classification 16S ribosomal RNA 040401 food science Bacterial communities 0104 chemical sciences Listeria Proteobacteria business Food Science Biotechnology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 12 pages, 6 figures, 3 tables The main aim of this study was to localise, identify and characterise the Listeria monocytogenes-harbouring bacterial communities present in food related premises via 16S rRNA gene metagenetic analysis. With this scope, 319 environmental samples coming from a wide variety of surfaces of fish (n = 120), meat (n = 80) and dairy industries (n = 119), were firstly analysed following ISO 11290–1 and ISO 11290–2 norms. Direct L. monocytogenes quantification was possible in 9 samples (2.8%) with values between 2.00 and 3.97 log CFU/cm2. After enrichment, an overall L. monocytogenes incidence of 12.54% (n = 40) was obtained, being samples from meat industry the most contaminated. Molecular serotyping assays showed that most of the isolates belonged to 1/2b-3b-7 subgroup, followed by 1/2a-3a and 1/2c-3c. These results combined with AscI and ApaI PFGE macrorestriction patterns, yielded 7 different L. monocytogenes clusters. Nevertheless, no clear ecological relationships could be stablished. High amounts of L. monocytogenes-associated psychrotrophic microbiota were obtained in all cases with values above 9 log CFU/cm2 in some cases. Metagenetic analysis of one representative sample per each food industry type (fish, meat, dairy) demonstrated that Actinobacteria (53.16%) was mostly present in the meat sample whereas Proteobacteria was the most representative phylum in dairy (69.58%) and fish (97.11%) samples. Subsequent operational taxonomic units (OTUs) analysis, showed a wide variety of taxa associated with L. monocytogenes such as spoilage-associated genera (e.g. Psyschromonas or Shewanella), lactic acid bacteria genera (e.g. Lactococcus or Lactobacillus) or pathogenic species such as Yersinia enterocolitica. It was thus demonstrated, that L. monocytogenes is capable to both survive with different bacteria in different ecological niches, highlighting once more the need for proper surveillance schedules so as to guarantee the safety of the food products This research was financially supported by the Spanish Ministerio de Economía, Industria y Competitividad [SOLISTA, AGL2016-78549] |
| Databáze: | OpenAIRE |
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