Co-transcription pattern of an introgressed operon in the maize chloroplast genome comprising four ATP synthase subunit genes and the ribosomal rps2
| Autor: | Steven R. Rodermel, Lawrence Bogorad, Alap R. Subramanian, Dietmar Stahl |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Ribosomal Proteins
Chloroplasts Transcription Genetic Operon Molecular Sequence Data Plant Science Genes Plant Zea mays Ribosomal protein Transcription (biology) Gene cluster Genetics Amino Acid Sequence Gene Adenosine Triphosphatases Base Sequence ATP synthase biology DNA General Medicine Ribosomal RNA Blotting Northern Chloroplast Multigene Family biology.protein Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 21:1069-1076 |
| ISSN: | 1573-5028 0167-4412 |
| Popis: | Several examples of the introduction of a gene from one gene complex into another (introgression) are found when chloroplast RP gene clusters are compared to those in Escherichia coli or cyanobacteria. Here we describe the transcript pattern of one such cluster from maize (Zea mays) that includes the genes for 4 subunits of the thylakoid ATP synthase (atpI, H, F, A) and the rps2 gene. Twelve transcript species covering the size range from 7,000 to 800 nt were identified in RNA isolated from dark-grown and greening maize seedlings, and several of them were characterized by reverse transcription analysis. A major species of 6,200 nt, with its 5' end at 181 nt upstream of the initiating ATG of rps2, contained the transcripts of all the 5 genes. Two further sets of transcripts having their 5' ends ca. 120 and 50 nt upstream of the initiation codons of the atpI and atpH genes were also identified. Thus, this plastid gene cluster in maize is functionally organized as an operon with additional regulatory features to allow for increased accumulation of mRNAs for the thylakoid components. |
| Databáze: | OpenAIRE |
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