Estimation of the Salmonella enterica prevalence in finishing swine
Autor: | R. D. Griffith, James D. McKean, H. S. Hurd, Marcos H. Rostagno |
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Jazyk: | angličtina |
Rok vydání: | 2004 |
Předmět: |
Salmonella
Veterinary medicine Epidemiology Swine animal diseases Enzyme-Linked Immunosorbent Assay medicine.disease_cause Sensitivity and Specificity Serology Specimen Handling Feces fluids and secretions Bias Ileum Risk Factors Seroepidemiologic Studies medicine Prevalence Animals Serotyping Cecum Swine Diseases Salmonella Infections Animal biology Salmonella enterica biology.organism_classification Antibodies Bacterial Infectious Diseases Population Surveillance Herd Lymph Autopsy Lymph Nodes Abattoirs Research Article |
Popis: | The study objective was to evaluate three methods of Salmonella enterica prevalence estimation in swine herds (faecal culture, culture of abattoir-collected samples, and serum ELISA). From each of six swine herds, we necropsied approximately 100 finishing pigs (>70 kg); one-half on farm and the other half at the abattoir, after transport and approximately 2·5 h holding. We collected the same samples for S. enterica culture at both locations (1 g faecal, 10 g caecal contents, ileocaecal lymph nodes, superficial inguinal lymph nodes, 25 g of gluteal muscle for serum ELISA). On farm, the 1 g faecal sample only detected 13·3% (2/15) of all positive pigs necropsied on farm. However, with abattoir and on-farm results combined, the faecal sample detected 57·4% (74/129) of positive pigs. Abattoir-collected samples provided prevalence estimates much higher than on-farm collected samples (39·9 vs. 5·3%; PS. enterica prevalence. For most herds, serology overestimated the on-farm culture prevalence. |
Databáze: | OpenAIRE |
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