Circulating extracellular vesicle-associated CD163 and CD206 in multiple myeloma

Autor: Marianne Hokland, Morten Andersen, Sarah L Kvorning, Holger Jon Møller, Niels Frost Andersen, Marlene Christina Nielsen
Rok vydání: 2019
Předmět:
Male
HUMAN MONOCYTES
Gene Expression
MICROENVIRONMENT
PROGRESSION
Gastroenterology
Monoclonal Gammopathy of Undetermined Significance
Polymerase Chain Reaction
ACTIVATION
0302 clinical medicine
Medicine
Receptors
Immunologic
Multiple myeloma
Whole blood
Membrane Glycoproteins
INDUCTION
FLOW-CYTOMETRY
Hematology
General Medicine
Extracellular vesicle
Blood Proteins
Plasma cell neoplasm
Middle Aged
Flow Cytometry
Blood proteins
multiple myeloma
TUMOR-ASSOCIATED MACROPHAGES
medicine.anatomical_structure
plasma cell neoplasms
030220 oncology & carcinogenesis
MONOCLONAL GAMMOPATHY
Female
molecular cytogenetics
extracellular vesicles
Multiple Myeloma
medicine.medical_specialty
tumour-associated macrophage
Antigens
Differentiation
Myelomonocytic
MANNOSE RECEPTOR
Receptors
Cell Surface
03 medical and health sciences
Extracellular Vesicles
Antigens
CD
Internal medicine
Humans
RNA
Messenger
Aged
Neoplasm Staging
IDENTIFICATION
business.industry
Monocyte
medicine.disease
CD206
Case-Control Studies
CD163
business
Monoclonal gammopathy of undetermined significance
Biomarkers
030215 immunology
Zdroj: Kvorning, S L, Nielsen, M C, Andersen, N F, Hokland, M, Andersen, M N & Møller, H J 2020, ' Circulating Extracellular Vesicle-associated CD163 and CD206 in Multiple Myeloma ', European Journal of Haematology, vol. 104, no. 5, pp. 409-419 . https://doi.org/10.1111/ejh.13371
ISSN: 1600-0609
Popis: Objectives Extracellular vesicles (EVs) are important for intercellular signalling in cancer. Tumour-associated macrophages, expressing the haemoglobin-haptoglobin and mannose receptors CD163 and CD206, are crucial for cancer progression. We recently identified CD163 on EVs in the circulation as a fraction of total soluble CD163 (sCD163). Here, we investigated the presence of CD163 and CD206-positive EVs (EV-CD163, EV-CD206) in patients with multiple myeloma (MM).Methods We enrolled patients with MM (n = 32), monoclonal gammopathy of undetermined significance (MGUS) (n = 8) and healthy donors (n = 16). Plasma protein levels were determined by ELISA before and after vesicle precipitation. Monocytes were examined by flow cytometry, and leucocyte CD163 mRNA by qPCR.Results Fractions of EV-CD163 and EV-CD206 were significantly elevated in patients with newly diagnosed MM (median = 39.8%, 76.5%, respectively) compared to patients with relapse (15.6%, P = .02, 42.5%, P = .003), remission (16.9%, P Conclusions Our results indicate that macrophage-derived EVs may play a role in the late phase of malignant progression of MM, and encourage further EV investigations in functional experiments.
Databáze: OpenAIRE
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