Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3
| Autor: | Lorne A. Babiuk, Alexandre N. Zakhartchouk, Neeraja Idamakanti, Joong Bok Lee, Caron Pyne, Mohit K. Baxi, P. Seshidhar Reddy, Suresh K. Tikoo |
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| Rok vydání: | 1998 |
| Předmět: |
Genes
Viral Transcription Genetic Sequence analysis viruses Molecular Sequence Data Immunology Sequence alignment Genome Viral Biology Microbiology Mastadenovirus Virology Animal Viruses Animals Humans Amino Acid Sequence ORFS Peptide sequence Gene Genomic organization Genetics Adenovirus genome Nucleic acid sequence Chromosome Mapping Molecular biology Insect Science Cattle Sequence Alignment |
| Zdroj: | Journal of Virology. 72:1394-1402 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.72.2.1394-1402.1998 |
| Popis: | The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. The deduced amino acid sequences of open reading frames (ORFs) in the late regions and early region 2 (E2) and for IVa2 show higher degrees of homology, whereas the predicted amino acid sequences of ORFs in the E1, E3, and E4 regions and the pIX, fiber, and 33,000-molecular-weight nonstructural proteins show little or no homology with the corresponding proteins of other adenoviruses. In addition, the penton base protein lacks the integrin binding motif, RGD, but has an LDV motif instead of an MDV motif. Interestingly, as in other animal adenoviruses, the virus-associated RNA genes appear to be absent from their usual location. Sequence analysis of cDNA clones representing the early- and late-region genes identified splice acceptor and splice donor sites, polyadenylation signals and polyadenylation sites, and tripartite leader sequences. |
| Databáze: | OpenAIRE |
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