Biosynthesis from gluconate of a random copolyester consisting of 3-hydroxybutyrate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. 61-3
| Autor: | Takeshi Fukushima, Hideki Abe, Hiroshi Eya, Yoshiharu Doi |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Gas Magnetic Resonance Spectroscopy 3-Hydroxybutyric Acid Polyesters Hydroxybutyrates General Medicine Nuclear magnetic resonance spectroscopy Gluconates Biochemistry Copolyester Gel permeation chromatography Polyester chemistry.chemical_compound Differential scanning calorimetry chemistry Structural Biology Pseudomonas Polymer chemistry Acetone Copolymer Molecular Biology Soil Microbiology Sodium gluconate |
| Zdroj: | International Journal of Biological Macromolecules. 16:115-119 |
| ISSN: | 0141-8130 |
| Popis: | Pseudomonas sp. 61-3 isolated from soil was found to produce a polyester consisting of 3-hydroxyalkanoic acids of even carbon numbers C4, C6, C8, C10 and C12 when sodium gluconate was fed as the sole carbon source. The polyester produced was fractionated with boiling acetone. The acetone-insoluble fraction (28 wt%) of the polyester was a poly(3-hydroxybutyrate) homopolymer, while the acetone-soluble fraction (72 wt%) was composed of seven different 3-hydroxyalkanoate (3HA) units ranging from C4 to C12: 40 mol% 3-hydroxybutyrate (3HB), 5 mol% 3-hydroxyhexanoate (3HH), 20 mol% 3-hydroxyoctanoate (3HO), 24 mol% 3-hydroxydecanoate (3HD), 1 mol% 3-hydroxy-5-cis-decenoate (3H5D), 4 mol% 3-hydroxydodecanoate (3HDD) and 6 mol% 3-hydroxy-5-cis- dodecenoate (3H5DD). The copolymer was characterized by nuclear magnetic resonance (NMR) spectroscopy, gel permeation chromatography and differential scanning calorimetry. The acetone-soluble fraction of this amorphous copolymer was shown to have a random sequence distribution of the seven 3HA units of C4 to C12 by analysis of the 150 MHz 13C-NMR spectrum. This is the first example of microbial synthesis of a random copolyester consisting of 3HB and medium-chain-length 3HA units. |
| Databáze: | OpenAIRE |
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