Intra- and inter-field diversity of 2,4-dichlorophenoxyacetic acid-degradative plasmids and theirtfdcatabolic genes in rice fields of the Mekong delta in Vietnam
| Autor: | Lars Hestbjerg Hansen, Dirk Springael, Søren J. Sørensen, Martin A. Hansen, Benjamin Horemans, Thi Phi Oanh Nguyen, René De Mot |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transposable element 2 4-Dichlorophenoxyacetic acid 030106 microbiology Context (language use) ORGANIZATION METABOLISM Biology Applied Microbiology and Biotechnology Microbiology PJP4 03 medical and health sciences chemistry.chemical_compound Plasmid Bacterial Proteins NUCLEOTIDE-SEQUENCE TRANSPOSON ADAPTATION Gene Soil Microbiology Genetics Science & Technology Ecology Herbicides Betaproteobacteria Oryza Interspersed Repetitive Sequences catabolic plasmid plasmid diversity SOIL Biodegradation Environmental 030104 developmental biology Vietnam chemistry 2 4-D biodegradation tfd genes BACTERIA HOST-RANGE PLASMIDS CHLOROPHENOL 2 4-Dichlorophenoxyacetic Acid Mobile genetic elements Life Sciences & Biomedicine local evolution Soil microbiology Plasmids |
| Zdroj: | Thi Phi Oanh Nguyen, Hansen, M A, Hansen, L H, Horemans, B, Sorensen, S J, De Mot, R & Springael, D 2019, ' Intra-and inter-field diversity of 2,4-dichlorophenoxyacetic acid-degradative plasmids and their tfd catabolic genes in rice fields of the Mekong delta in Vietnam ', FEMS Microbiology Ecology, vol. 95, no. 1, 214 . https://doi.org/10.1093/femsec/fiy214 |
| ISSN: | 1574-6941 |
| DOI: | 10.1093/femsec/fiy214 |
| Popis: | The tfd genes mediating degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) differ in composition and organization in bacterial isolates from different geographical origin and are carried by different types of mobile genetic elements (MGE). It is not known whether such global diversity of 2,4-D-catabolic MGE and their tfd gene cargo is reflected in the diversity at field scale. The genomic context of the 2,4-D catabolic genes of 2,4-D-degrading isolates from two rice fields with a 2,4-D application history, located in two distant provinces of the Vietnam Mekong delta, was compared. All isolates were β-proteobacteria, were unique for each rice field and carried the catabolic genes on MGE and especially plasmids. Most plasmids were IncP-1β plasmids and carried tfd clusters highly similar to those of the IncP-1β plasmid pJP4, typified by two chlorophenol catabolic gene modules (tfd-I and tfd-II). IncP-1β plasmids from the same field showed small deletions and/or insertions in accessory metabolic genes. One plasmid belonged to an unclassified plasmid group and carries a copy of both tfdA and tfd-II identical to those in the IncP-1β plasmids. Our results indicate intra-field evolution and inter-field exchange of 2,4-D-catabolic IncP-1β plasmids as well as the exchange of tfd genes between different plasmids within a confined local environment. ispartof: FEMS MICROBIOLOGY ECOLOGY vol:95 issue:1 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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