Glycine Codon Discrimination and the Nucleotide inPosition 32 of the Anticodon Loop
| Autor: | Miroslawa Z. Barciszewska, Catharina Claesson, Ulf Lagerkvist, Carolina Simonsson, Thomas Borén, Florentyna Lustig |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
chemistry.chemical_classification Base Sequence Molecular Sequence Data Glycine Wild type Mycoplasma mycoides RNA Transfer Gly Wobble base pair Biology Biochemistry chemistry Structural Biology Protein Biosynthesis Mutation Transfer RNA Anticodon Nucleotide Codon Molecular Biology Glycine tRNA |
| Zdroj: | Journal of Molecular Biology. 247:191-196 |
| ISSN: | 0022-2836 |
| Popis: | Using an in vitro protein-synthesizing system that allowed us to monitor separately the reading of each glycine codon, we have previously shown, that in constructs based on glycine tRNA1 from Escherichia coli the nature of the nucleotide in position 32 determines the ability of the anticodon UCC to discriminate between the glycine codons. Thus, with a U in position 32 the anticodon UCC discriminated according to the wobble rules, but with a C in this position it had lost its ability to discriminate. In the present paper we show that the same is true also for constructs based on mycoplasma glycine tRNA. When C32 in the wild type was changed to U32, the anticodon UCC discriminated between the glycine codons, while in wild type mycoplasma glycine tRNA it did not. Furthermore, when U32 was changed to C32 in glycine tRNA1(CCC), the anticodon CCC loses its ability to discriminate. We therefore conclude that the nature of the nucleotide in position 32 determines the discriminatory ability of both anticodons UCC and CCC in the glycine tRNA1 structural background, and that the same is true for the anticodon UCC in the mycoplasma glycine tRNA background. |
| Databáze: | OpenAIRE |
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